Moderate stress responses and specific changes in polyamine metabolism characterize Scots pine somatic embryogenesis

Tree Physiology
Heikki M SaloJaana Vuosku

Abstract

Somatic embryogenesis (SE) is one of the methods with the highest potential for the vegetative propagation of commercially important coniferous species. However, many conifers, including Scots pine (Pinus sylvestris L.), are recalcitrant to SE and a better understanding of the mechanisms behind the SE process is needed. In Scots pine SE cultures, embryo production is commonly induced by the removal of auxin, addition of abscisic acid (ABA) and the desiccation of cell masses by polyethylene glycol (PEG). In the present study, we focus on the possible link between the induction of somatic embryo formation and cellular stress responses such as hydrogen peroxide protection, DNA repair, changes in polyamine (PA) metabolism and autophagy. Cellular PA contents and the expression of the PA metabolism genes arginine decarboxylase (ADC), spermidine synthase (SPDS), thermospermine synthase (ACL5) and diamine oxidase (DAO) were analyzed, as well as the expression of catalase (CAT), DNA repair genes (RAD51, KU80) and autophagy-related genes (ATG5, ATG8) throughout the induction of somatic embryo formation in Scots pine SE cultures. Among the embryo-producing SE lines, the expression of ADC, SPDS, ACL5, DAO, CAT, RAD51, KU80 and ATG8 showed ...Continue Reading

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Citations

Apr 30, 2017·International Journal of Radiation Biology·Mandar Sengupta, Sarmistha Sen Raychaudhuri
Mar 1, 2018·Plant & Cell Physiology·Leandro Francisco de OliveiraEny Iochevet Segal Floh
Feb 23, 2019·Frontiers in Plant Science·Hugo A Méndez-HernándezVíctor M Loyola-Vargas
Mar 14, 2021·Plant Physiology and Biochemistry : PPB·Neidiquele M SilveiraEduardo C Machado
May 1, 2021·Plant Cell Reports·Siew-Eng OoiMeilina Ong-Abdullah

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