Modern Analysis of Protein Folding by Differential Scanning Calorimetry

Methods in Enzymology
Beatriz Ibarra-MoleroVictor Muñoz

Abstract

Differential scanning calorimetry (DSC) is a very powerful tool for investigating protein folding and stability because its experimental output reflects the energetics of all conformations that become minimally populated during thermal unfolding. Accordingly, analysis of DSC experiments with simple thermodynamic models has been key for developing our understanding of protein stability during the past five decades. The discovery of ultrafast folding proteins, which have naturally broad conformational ensembles and minimally cooperative unfolding, opens the possibility of probing the complete folding free energy landscape, including those conformations at the top of the barrier to folding, via DSC. Exploiting this opportunity requires high-quality experiments and the implementation of novel analytical methods based on statistical mechanics. Here, we cover the recent exciting developments in this front, describing the new analytical procedures in detail as well as providing experimental guidelines for performing such analysis.

Citations

Dec 8, 2017·PloS One·Valeriya V MikhaylovaBoris I Kurganov
Aug 31, 2016·The Biochemical Journal·Victor Muñoz, Michele Cerminara
Aug 17, 2016·The Biochemical Journal·M Luisa Romero-RomeroJose M Sanchez-Ruiz
Mar 15, 2018·Nucleic Acids Research·Sneha MunshiAthi N Naganathan
May 16, 2021·Ultrasonics Sonochemistry·Xuyue WangShugang Li
Jun 19, 2021·The Journal of Physical Chemistry. B·Daniel Mark ShapiroAshutosh Chilkoti
Dec 20, 2016·Journal of the American Chemical Society·Abhishek NarayanAthi N Naganathan
Apr 20, 2019·Biochemistry·Sneha MunshiAthi N Naganathan
Jul 27, 2018·The Journal of Physical Chemistry. B·Abhishek Narayan, Athi N Naganathan
Jul 10, 2018·The Journal of Physical Chemistry. B·Manuel Iglesias-BexigaIrene Luque
Sep 22, 2020·The Journal of Physical Chemistry. B·Sandhyaa SubramanianAthi N Naganathan
Dec 6, 2021·Protein Science : a Publication of the Protein Society·Sina KordesBirte Höcker

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