PMID: 20650127Aug 1, 1995Paper

Modern biostatistical methods for assessing in vitro/in vivo correlation of severely eye irritating chemicals in a validation study of in vitro alternatives to the Draize eye test

Toxicology in Vitro : an International Journal Published in Association with BIBRA
H SpielmannO de Silva

Abstract

The HET-CAM test and 3T3 cell neutral red uptake (NRU) cytotoxicity assay were evaluated in a national German validation project to replace the Draize eye test for classifying severely eye irritating chemicals, which have to be labelled 'R-41' according to EU regulations. As testing of 200 chemicals in the two in vitro assays did not sufficiently allow severely eye irritating chemicals to be identified and since the scoring system of the HET-CAM assay has been derived empirically, it was investigated whether modern biostatistical methods, for example discriminant analysis, would improve the selection of predictive endpoints of the HET-CAM assay. Comparison of HET-CAM data with adverse reactions observed in different tissues of the rabbit's eye proved that complex regression models are better describing in vitro /in vivo correlations than simple linear models. Discriminant analysis revealed that among the nine endpoints routinely determined in the HET-CAM test, coagulation was the only acceptable endpoint to classify severely irritating chemicals 'R-41' according to EU regulations. To identify R-41 chemicals the reaction time of appearance of coagulation of a 10% solution was the best discriminating factor and coagulation of the...Continue Reading

References

Feb 1, 1985·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·N P Luepke
Jan 1, 1991·Toxicology in Vitro : an International Journal Published in Association with BIBRA·H SpielmannF J Wiebel
Jul 1, 1993·Toxicology in Vitro : an International Journal Published in Association with BIBRA·H SpielmannW Steiling

❮ Previous
Next ❯

Citations

Jan 1, 1997·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·H SpielmannW Steiling
Nov 6, 2003·Toxicology in Vitro : an International Journal Published in Association with BIBRA·W WongK L Moran
Jun 1, 1998·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M H AbrahamW S Cain
Feb 1, 2006·Environmental Toxicology and Pharmacology·Sandra CoeckeThomas Hartung
Feb 8, 2011·Reproductive Toxicology·Marianne BarrierE Sidney Hunter
Jul 6, 2010·Regulatory Toxicology and Pharmacology : RTP·Mark H KleinmanChris D Geddes
Dec 4, 2012·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Franzisca Marie BätzMonika Schäfer-Korting
Mar 25, 2005·Physiological Reviews·Anna M Wobus, Kenneth R Boheler
Mar 13, 2008·Indoor Air·M G ApteM J Mendell
May 15, 2008·International Journal of Cancer. Journal International Du Cancer·Lin FritschiNicholas de Klerk
Oct 15, 2011·Journal of Hospital Medicine : an Official Publication of the Society of Hospital Medicine·Michael B RothbergMarjorie Keyes
Oct 1, 2005·Alternatives to Laboratory Animals : ATLA·Chantra EskesValérie Zuang
Dec 15, 2004·Alternatives to Laboratory Animals : ATLA·Lutz Edler, Carina Ittrich
Sep 9, 2010·Alternatives to Laboratory Animals : ATLA·Yang YingGuo Xiang
Feb 6, 2021·In Vitro Cellular & Developmental Biology. Animal·Yulia Kaluzhny, Mitchell Klausner

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cavernous Hemangioma

Cavernous hemangioma is a blood vessel defect or benign tumor that leads to leakage of blood to the surrounding tissues. This can occur in several organs including the brain, which can lead to seizures. Discover the latest research on cavernous hemangiomas here.

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.

Related Papers

Toxicology in Vitro : an International Journal Published in Association with BIBRA
S KalweitH Spielmann
Toxicology in Vitro : an International Journal Published in Association with BIBRA
M BallsH Spielmann
Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
W A ChambersP T Liu
© 2021 Meta ULC. All rights reserved