Modern field emission scanning electron microscopy provides new perspectives for imaging kidney ultrastructure

Kidney International
Carsten DittmayerSebastian Bachmann

Abstract

Recent progress in electron microscopy (EM) techniques has opened new pathways to study renal tissue in research and pathology. Modern field emission scanning EM may be utilized to scan thin sections of resin-embedded tissue mounted on a conductive support. Here we sought to achieve automated imaging without the typical limitations of transmission EM with equivalent or superior quality. Extended areas of tissue were either imaged in two (nanotomy) or in three dimensions (volume EM) by serial-section-based array tomography. Single-beam and fast-recording multi-beam field emission scanning EM instruments were compared using perfusion-fixed rodent kidneys. High-resolution scans produced excellent images of tissue, cells, and organelles down to macromolecular complexes. Digital stitching of image tiles in both modes allowed seamless Google Earth-like zooming from overview to regions of interest at the nanoscale. Large datasets were created that can be rapidly shared between scientists of different disciplines or pathologists using open source software. Three-dimensional array tomography of thin sections was followed by segmentation to visualize selected features in a large volume. Furthermore, correlative light-EM enabled the ident...Continue Reading

Citations

Nov 17, 2020·American Journal of Physiology. Renal Physiology·Yan XuSebastian Bachmann
Mar 30, 2021·Frontiers in Cell and Developmental Biology·Yuto KawasakiKoichiro Ichimura
Jul 17, 2021·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Carsten DittmayerSebastian Bachmann

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