PMID: 3746892Jan 1, 1986Paper

Modes of Cl- transport across the mucosal and serosal membranes of urodele intestinal cells

The Journal of Membrane Biology
J F White

Abstract

The characteristics of Cl- movement across luminal and basolateral membranes of Amphiuma intestinal absorptive cells were studied using Cl(-)-sensitive microelectrodes and tracer 36Cl techniques. Intracellular Cl- activity (aiCl) was unchanged when serosal Cl- was replaced; when luminal Cl- was replaced cell Cl- was rapidly lost. Accordingly, the steady state aiCl could be varied by changing the luminal [Cl]. As luminal [Cl] was raised from 1 to 86 mM, aiCl rose in a linear manner, the mucosal membrane hyperpolarized, and the transepithelial voltage became serosa negative. In contrast, the rate of Cl- transport from the cell into the serosal medium, measured as the SITS-inhibitable portion of the Cl- absorptive flux, attained a maximum when aiCl reached an apparent value of 17 mM, indicating the presence of a saturable, serosal transport step. The stilbene-insensitive absorptive flux was linear with luminal [Cl], suggestive of a paracellular route of movement. Intracellular aCl was near electrochemical equilibrium at all but the lowest values of luminal [Cl] after interference produced by other anions was taken into account. aiCl was unaffected by Na replacement, removal of medium K, or elevation of medium HCO-3. Mucosae labele...Continue Reading

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Citations

Jan 1, 1991·Comparative Biochemistry and Physiology. A, Comparative Physiology·D SaidaneG Peres
Jan 1, 1988·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·R G Smith
Mar 11, 2006·Evolution; International Journal of Organic Evolution·Ana LlopartJerry A Coyne
May 1, 1989·The American Journal of Physiology·J F White, D Ellingsen
Aug 1, 1989·The American Journal of Physiology·J F White
May 1, 1987·The American Journal of Physiology·J F WhiteS Mayer

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