Modification by glucose of the flocculent phenotype of a Kloeckera apiculata wine strain.

Journal of Industrial Microbiology & Biotechnology
Oscar A SosaMarta E Farías

Abstract

We have evaluated the induction of the flocculent phenotype of Kloeckera apiculata by glucose mc1 and propose a pathway involved in carbohydrate flocculation induction. Pulses of glucose were given to cells growing in glucose-poor medium (2 g l(-1)) and the flocculation percentage was measured. To elucidate the mechanism involved in flocculation induction, cycloheximide was injected into the cultures 120 min before the glucose pulse. 2,4-Dinitrophenol or cAMP was added to the media instead, or simultaneously with glucose, while a protein kinase A (PKA) inhibitor was added 30 min before the glucose pulse. With 20 and 50 g l(-1) glucose pulse, the yeast flocculation percentage arises to 55 and 65%, respectively. The quantity of proteins and the reflocculating capacity of a lectinic protein extract from the yeast cell wall increase as the concentration of glucose pulse was higher. Cycloheximide prevented the glucose-induced flocculation, while cAMP or 2,4-dinitrophenol increased it 4- and 5-fold, respectively. PKA inhibitor completely prevented the glucose induction flocculation. The flocculent phenotype of K. apiculata mc1 was induced by glucose and the mechanism seems to imply de novo protein (lectin) synthesis via the PKA trans...Continue Reading

References

Jan 1, 1992·Biotechnology & Genetic Engineering Reviews·M Stratford
Aug 1, 1994·Applied and Environmental Microbiology·M H StraverJ W Kijne
Aug 1, 1993·Yeast·P A FernandesP Moradas-Ferreira
Apr 1, 1993·Yeast·M Stratford, A T Carter
Jun 1, 1996·Canadian Journal of Microbiology·E V Soares, M Mota
Jan 31, 2003·Molecular and Cellular Biology·Valmik K VyasMarian Carlson
Jul 16, 2003·Journal of Applied Microbiology·E V Soares, A Vroman
Aug 2, 2003·International Journal of Food Microbiology·P RomanoA Capece
Aug 13, 2003·Journal of Applied Microbiology·M E Farías, M C Manca de Nadra
Sep 29, 2004·Trends in Biotechnology·Kevin J VerstrepenFreddy R Delvaux
Dec 1, 2004·Annual Review of Genetics·Monica A Schwartz, Hiten D Madhani
Jan 22, 2005·Journal of Applied Microbiology·S SampermansE V Soares
Mar 25, 2006·Molecular Microbiology·Kevin J Verstrepen, Frans M Klis

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Citations

Mar 29, 2014·World Journal of Microbiology & Biotechnology·Claudia Lorena Fernandez LopezPatricia Taillandier
Feb 3, 2016·Frontiers in Microbiology·Warren AlbertinIsabelle Masneuf-Pomarede
Jun 13, 2015·Critical Reviews in Food Science and Nutrition·Youzhong LiuHervé Alexandre

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