Modification of a supported lipid bilayer by polyelectrolyte adsorption

Langmuir : the ACS Journal of Surfaces and Colloids
Z Vivian FengAndrew A Gewirth

Abstract

Addition of a weak polyelectrolyte, poly(methacrylic acid) (PMA), to a supported phospholipid bilayer made from 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) depresses the melting temperature and alters the morphology of the bilayer in the gel phase. Ellipsometry measurements show that PMA adsorption lowers the phase transition temperature by 2.4 degrees C. Atomic force microscopy (AFM) showed no visible contrast in the fluid phase (above the melting temperature) but a rich morphology in the gel phase. In the gel phase, adsorption leads to formation of significantly less mobile phospholipid islands and other defects. One consequence of this lower mobility is a decrease in the implied cooperativity number of the phase transition, N, when polymer is added. Additionally, AFM images of the gel-phase bilayer show a highly defected structure that anneals significantly more slowly than in the absence of adsorbed polymer. Tentatively, we suggest that PMA preferentially decorates island and defect edges of the DMPC bilayer.

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Citations

Oct 7, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Emily A SmithMary J Wirth
Jun 2, 2005·Chemical Communications : Chem Comm·Gopal KrishnaYuri Lvov
Sep 7, 2006·Physical Chemistry Chemical Physics : PCCP·Ryugo TeroTsuneo Urisu
Nov 9, 2006·Annual Review of Physical Chemistry·Sung Chul Bae, Steve Granick
Aug 10, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Julio H K RozenfeldM Teresa Lamy
Dec 26, 2015·Membranes·Martin KreuzerRoland Steitz
Sep 2, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Ignacio F GallardoIlya J Finkelstein
Feb 24, 2015·Physical Chemistry Chemical Physics : PCCP·Julio H K RozenfeldM Teresa Lamy
Jun 13, 2019·The Journal of Membrane Biology·T DevanandSatyavani Vemparala
May 2, 2018·Biochimica Et Biophysica Acta. Biomembranes·Pouyan Khakbaz, Jeffery B Klauda
Oct 23, 2008·Environmental Science & Technology·Andrew S CampbellAndrew A Gewirth
Mar 15, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Andrey FilippovStaffan Tavelin
Feb 14, 2006·The Journal of Physical Chemistry. B·Liangfang ZhangSteve Granick
Feb 15, 2007·Nano Letters·Tighe A Spurlin, Andrew A Gewirth

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