Modification of an Anopheles gambiae odorant binding protein to create an array of chemical sensors for detection of drugs.

Scientific Reports
K Cali, K C Persaud

Abstract

The binding pockets of odorant binding proteins from Anopheles gambiae (OBP1 and OBP47) were analysed using in silico modelling. The feasibility of creating mutant proteins to achieve a protein array capable of detecting drugs of abuse in solution or in vapour phase was investigated. OBP1 was found to be easily adapted and several mutant proteins were expressed and characterised. AgamOBP1_S82P was found to have high affinities to cannabinol, 3,4-methylenedioxy methamphetamine (MDMA/Ecstasy) and cocaine hydrochloride. When these proteins were immobilised on a quartz crystal microbalance, saturated cocaine hydrochloride vapour could be detected. The sensors were stable over a period of at least 10 months in air. The approach taken allows flexible design of new biosensors based on inherently stable protein scaffolds taking advantage of the tertiary structure of odorant binding proteins.

References

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Citations

Sep 6, 2020·Applied Microbiology and Biotechnology·Nathália F BritoAna Claudia A Melo
Oct 3, 2020·Scientific Reports·Cyril HamiauxJacob A Corcoran
Jan 22, 2021·Cell and Tissue Research·Matthew WheelwrightOlena Riabinina

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Methods Mentioned

BETA
biosensor
biosensors
X-ray
electrophoresis
fluorescence displacement
quartz crystal microbalance
quartz crystal microbalances
gel filtration

Software Mentioned

PoPMuSiC
KINARI MUTAGEN
Computed Atlas of Surface Topography of proteins ( CASTp )
Mutagen tools
Jmol
KINARI
PoPMuSiC ( Prediction
KINARI MUTAGEN SERVER
MSD ChemStation
MassHunter GC / MS

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