PMID: 9188096Mar 1, 1997Paper

Modification of annexin II expression in PC12 cell lines does not affect Ca(2+)-dependent exocytosis

Molecular Biology of the Cell
M E GrahamR D Burgoyne

Abstract

The Ca2+/phospholipid/cytoskeletal-binding protein annexin II has been proposed to play an important role in Ca(2+)-dependent exocytosis; however, the evidence for this role is inconclusive. More direct evidence obtained by manipulating annexin II levels in cells is still required. We have attempted to do this by generating stably transfected PC12 cell lines expressing proteins which elevate or lower functional annexin II levels and using these cell lines to investigate Ca(2+)-dependent exocytosis. Three cell lines were generated: one expressing an annexin II mutant which aggregates annexin II in at least a proportion of the cells, thereby removing functional protein from the cell; a mixed clonal cell line constitutively overexpressing human annexin II; and a clonal cell line capable of over-expressing annexin II in the presence of sodium butyrate. After digitonin permeabilization, Ca(2+)-dependent dopamine release from these cell lines was compared with that from control nontransfected cells, and, in addition, release was compared in induced to uninduced cells. There were no significant differences in Ca(2+)-dependent exocytosis between any of the transfected cell lines before or after induction and the control cells. In addit...Continue Reading

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Citations

Aug 26, 2000·Progress in Biophysics and Molecular Biology·J I Kourie, H B Wood
Jun 2, 2005·Nature Reviews. Molecular Cell Biology·Volker GerkeStephen E Moss
Aug 7, 1998·Molecular Biology of the Cell·L H Chamberlain, R D Burgoyne
Jan 7, 2005·Molecular Biology of the Cell·Sylvette Chasserot-GolazMarie-France Bader
Sep 23, 1998·The Journal of Cell Biology·F LafontK Simons
Aug 18, 2001·Current Biology : CB·C J MerrifieldS E Moss
Apr 3, 2016·Journal of Neurochemistry·Marion Gabel, Sylvette Chasserot-Golaz
Mar 26, 1999·Cell Calcium·R D Burgoyne, A Morgan
Mar 21, 1998·The Journal of Biological Chemistry·D B ZimmerC M Donald
Mar 23, 2013·International Journal of Molecular Sciences·Alamelu BharadwajDavid Waisman
Oct 23, 2003·The Journal of Biological Chemistry·Lee P HaynesRobert D Burgoyne
Jun 22, 2002·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Torbjørn Hansen
Dec 3, 1999·Journal of Neurochemistry·P WashbourneC Montecucco
Apr 6, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·A ChanderA R Spitzer
Dec 14, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·B M FreyF J Frey

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