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Modification of arginine and lysine in proteins with 2,4-pentanedione

Biochemistry

Nov 18, 1975

Hiram F Gilbert, Marion H. O'Leary

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Abstract

Primary amines react with 2,4-pentanedione at pH 6-9 to form enamines, N-alkyl-4-amino-3-penten-2-ones. The latter compounds readily regenerate the primary amine at low pH or on treatment with hydroxylamine. Guanidine and substituted guanidines react with 2,4-pentanedione to form N-subs...read more

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Ketones
Leftose
Lysine
Lysozyme
Guanidines
Arginine hydrochloride
Lysozyme Test
Enisyl
Guanidine
Muramidase
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  • Modification of arginine and lysine in proteins with 2,4-pentanedione

    Biochemistry

    Nov 18, 1975

    Hiram F Gilbert, Marion H. O'Leary

    PMID: 43

    DOI: 10.1021/bi00694a027

    Abstract

    Primary amines react with 2,4-pentanedione at pH 6-9 to form enamines, N-alkyl-4-amino-3-penten-2-ones. The latter compounds readily regenerate the primary amine at low pH or on treatment with hydroxylamine. Guanidine and substituted guanidines react with 2,4-pentanedione to form N-subs...read more

    Mentioned in this Paper

    Ketones
    Leftose
    Lysine
    Lysozyme
    Guanidines

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