PMID: 2123717Sep 11, 1990Paper

Modification of bases in DNA by copper ion-1,10-phenanthroline complexes

Biochemistry
M DizdarogluB Halliwell

Abstract

Damage to the bases in DNA by the cupric ion-1,10-phenanthroline complex was investigated. Ten base products in DNA were identified and quantitated by the use of gas chromatography/mass spectrometry with selected-ion monitoring. DNA damage by the cupric ion-1,10-phenanthroline complex required the presence of a reducing agent such as ascorbic acid or mercaptoethanol. Products identified were typical hydroxyl radical induced products from the pyrimidines and purines in DNA, well-known from previous studies using various hydroxyl radical producing systems such as ionizing radiation, hypoxanthine/xanthine oxidase, or hydrogen peroxide in the presence of transition metal ions. Product formation was not significantly inhibited by typical scavengers of hydroxyl radical such as mannitol and sodium formate, but there was partial inhibition by dimethyl sulfoxide. Catalase substantially decreased formation of base products, and added hydrogen peroxide stimulated it, indicating the hydrogen peroxide dependency of DNA base damage. Superoxide dismutase afforded only a partial reduction in product yields in systems containing ascorbic acid. On the basis of the types of base products formed, the hydrogen peroxide dependency of product formati...Continue Reading

References

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Citations

Apr 6, 2001·International Journal of Cancer. Journal International Du Cancer·Y HirakuS Kawanishi
Sep 8, 1993·Molecular and Cellular Biochemistry·M CalderaroR Meneghini
Feb 9, 2007·Cancer Chemotherapy and Pharmacology·Radamés Alemón-MedinaLena Ruiz-Azuara
Apr 9, 2005·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Jiuhong KangZhenhua Wang
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Jan 1, 1991·Free Radical Biology & Medicine·M Dizdaroglu
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Sep 11, 2002·Archives of Biochemistry and Biophysics·Katsuhisa Sakano, Shosuke Kawanishi
Sep 5, 2002·Free Radical Biology & Medicine·Katsuhisa SakanoShosuke Kawanishi
Sep 2, 2003·Mutation Research·Kazuhiko OgawaShosuke Kawanishi
Sep 12, 2013·Chemical Research in Toxicology·Mariko MurataShosuke Kawanishi
Aug 2, 2003·Archives of Biochemistry and Biophysics·Takuya IwamotoShosuke Kawanishi
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Mar 5, 1991·Biochemistry·E GajewskiB Halliwell
Dec 24, 2018·Free Radical Research·Yurie MoriShinji Oikawa

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