PMID: 8596690Feb 1, 1996Paper

Modification of C-type inactivating Shaker potassium channels by chloramine-T

Pflügers Archiv : European journal of physiology
T SchliefS H Heinemann

Abstract

Shaker potassium channels undergo a slow C-type inactivation which can be hastened dramatically by single-point mutations in or near the pore region. We found that the oxidizing agent chloramine-T (Chl-T) causes an irreversible loss of current for those mutants which show C-type inactivation. For several mutants at position T449, which show a wide spectrum of inactivation time constants, the time constant of current rundown induced by Chl-T correlated with the speed of inactivation. Rundown was accelerated when the channels were in the inactivated state but rundown also occurred when channels were not opened or inactivated. Apparently, only those channels which can undergo C-type inactivation are accessible to Chl-T. In order to gain information about the target amino-acid residue for the action of Chl-T and the structural rearrangements occurring during C-type inactivation, several mutant channel proteins were compared with respect to their response to Chl-T. Since Chl-T can oxidize cysteine and methionine residues, we mutated the possible targets in and close to the pore region, namely C462 to A, and M440 and M448 to I. While the residues M440 and C462 were not important for channel rundown, mutation of M448 to I made the cha...Continue Reading

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Citations

Apr 1, 1997·The Journal of General Physiology·C MathesW F Gilly
Sep 8, 2006·American Journal of Physiology. Cell Physiology·Cyrus EduljeeSteven J Kehl
Mar 29, 2008·Pflügers Archiv : European journal of physiology·Mario KassmannStefan H Heinemann
Apr 30, 1998·Circulation Research·R L RasmussonH C Strauss
Nov 14, 1997·The Journal of General Physiology·J G StarkusS H Heinemann
Jul 3, 1998·The Journal of General Physiology·J G StarkusS H Heinemann
Feb 27, 2001·The Journal of General Physiology·X D TangT Hoshi
Sep 2, 1997·Proceedings of the National Academy of Sciences of the United States of America·M A CiorbaT Hoshi
Sep 1, 1996·Pflügers Archiv : European journal of physiology·C K BauerJ R Schwarz
Apr 15, 2006·Molecular Pharmacology·Klara SusankovaViktorie Vlachova
Jul 9, 2010·Nature·Luis G CuelloEduardo Perozo
Aug 19, 2011·Journal of Neurophysiology·Alfredo J GarciaJan-Marino Ramirez
May 28, 2013·Pflügers Archiv : European journal of physiology·Yen May ChengSteven J Kehl
Sep 18, 2013·Antioxidants & Redox Signaling·Nirakar SahooStefan H Heinemann
Jun 15, 2016·Proceedings of the National Academy of Sciences of the United States of America·Jiusheng YanRichard W Aldrich
Jan 16, 2013·The Journal of General Physiology·Toshinori Hoshi, Clay M Armstrong
Dec 1, 2005·Progress in Biophysics and Molecular Biology·Harley T Kurata, David Fedida
Mar 5, 2003·Medical Hypotheses·Thomas W Stief
Nov 6, 2018·The Journal of Physical Chemistry. B·Hiroko X KondoKengo Kinoshita

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