Modification of carboxyl groups in botulinum neurotoxin types A and E

Toxicon : Official Journal of the International Society on Toxinology
M A WoodyB R DasGupta

Abstract

Effects of chemical modification of carboxyl groups of botulinum neurotoxin serotypes A and E were studied by using a water soluble carbodiimide-nucleophile reaction that is highly specific for modifying carboxyl groups of proteins. In both types A and E, increasing levels of the reagents, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide and norleucine methyl ester or glycine methyl ester, at pH 4.8 caused increased loss of toxicity. More glycine could be incorporated than norleucine. Amino acid analysis did not reveal modification of any amino acid residue other than carboxyl groups (possible reaction of sulfhydryl groups was not studied). Loss of one carboxyl group did not severely affect toxicity, but modification of three carboxyl groups caused greater than 95% detoxification in both types. Complete detoxification could not be achieved with any amount of the reagents. Modification of three to five carboxyl groups did not affect serological activity.

References

Mar 15, 1979·Biochemical and Biophysical Research Communications·A L George, C L Borders
Feb 21, 1989·Molecular and Cellular Biochemistry·M Woody, B R DasGupta
Sep 1, 1988·Molecular and Cellular Biochemistry·V Sathyamoorthy, B R DasGupta
Oct 1, 1972·Infection and Immunity·B R Das Gupta, H Sugiyama
Aug 21, 1970·Biochimica Et Biophysica Acta·J KnoxL Spero
Jul 11, 1972·Biochemical and Biophysical Research Communications·B R DasGupta, H Sugiyama
Jan 1, 1983·Toxicon : Official Journal of the International Society on Toxinology·B R DasGupta, S Rasmussen
Jan 1, 1984·Toxicon : Official Journal of the International Society on Toxinology·B R DasGupta, V Sathyamoorthy
Jul 1, 1984·Archives of Biochemistry and Biophysics·B R Dasgupta, S Rasmussen
Mar 28, 1980·Biochemical and Biophysical Research Communications·B R DasGupta, H Sugiyama
Jul 31, 1980·Biochemical and Biophysical Research Communications·J W Reinsch, R B Dunlap
Aug 31, 1981·Biochemical and Biophysical Research Communications·B R DasGupta, S Rasmussen
Jan 1, 1972·Methods in Enzymology·K L Carraway, D E Koshland

❮ Previous
Next ❯

Related Concepts

Related Feeds

Botulism (ASM)

Botulism is a rare but serious paralytic illness caused by a nerve toxin that is produced by the bacterium clostridium botulinum. Discover the latest research on botulism here.

Botulism

Botulism is a rare but serious paralytic illness caused by a nerve toxin that is produced by the bacterium clostridium botulinum. Discover the latest research on botulism here.

Related Papers

Methods in Enzymology
K L Carraway, D E Koshland
Toxicon : Official Journal of the International Society on Toxinology
V M MahnirG B Elyakov
Toxicon : Official Journal of the International Society on Toxinology
B W LennonI I Kaiser
© 2022 Meta ULC. All rights reserved