PMID: 9630847Jun 19, 1998Paper

Modification of cell cycle and viability of TLX5 lymphoma in vitro by sulfoxide-ruthenium compounds and cisplatin detected by flow cytometry

Chemico-biological Interactions
I CapozziG Sava

Abstract

The effects of Na[trans-RuCl4(DMSO)Im] (NAMI), Na[trans-RuCl4(TMSO) Ind] (TIND) and Na[trans-RuCl4(TMSO)Iq] TEQU) were tested in vitro on TLX5 lymphoma cells in comparison to cisplatin by means of the sulforhodamine-B test SRB) for protein content determination, by acridine orange and propidium iodide staining and by means of the bromodeoxyuridine test, for cell cycle modifications. After 1 h drug exposure with metal-based drugs, TLX5 lymphoma cells require a further 72 h in vitro cultivation to show alteration of cell cycle. Ruthenium compounds show a different pattern of effects: TEQU causes the same dose-dependent cytotoxicity and DNA fragmentation shown by cisplatin, TIND reduces absorbance with the SRB test and slightly increases S and G2M populations with a time-dependent drug exposure of tumour cells, and NAMI is virtually devoid of any detectable effect. By in vivo bioassay of in vitro treated tumour cells, TIND and TEQU are effective independently of the time of drug exposure of tumour cells, this effect being confirmed by the same cell uptake of ruthenium after 1 or 4 h treatment, determined by atomic absorption spectroscopy. These data stress the lack of the involvement of direct cytotoxic effects in the potent anti-...Continue Reading

References

Jul 4, 1990·Journal of the National Cancer Institute·P SkehanM R Boyd
Nov 1, 1989·Clinical & Experimental Metastasis·G SavaT Giraldi
Mar 1, 1994·Clinical & Experimental Metastasis·R GagliardiE Alessio
Jan 1, 1996·Breast Cancer Research and Treatment·M R SchneiderH Graf
Sep 27, 1996·International Journal of Cancer. Journal International Du Cancer·G SavaS Garbisa

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Citations

Sep 10, 2010·Journal of Biosciences·Aliny Pereira de LimaElisângela de Paula Silveira-Lacerda
Dec 7, 2011·Biological Trace Element Research·Aliny Pereira de LimaElisângela de Paula Silveira-Lacerda
Jul 6, 2010·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Aliny Pereira de LimaElisângela de Paula Silveira Lacerda
Nov 4, 2000·Expert Opinion on Investigational Drugs·D R Richardson, D B Lovejoy
Nov 5, 2016·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Maria OszajcaMałgorzata Brindell
Mar 29, 2014·Journal of Medicinal Chemistry·Catherine M ClavelPaul J Dyson

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