PMID: 9188095Mar 1, 1997Paper

Modification of Cys-837 identifies an actin-binding site in the beta-propeller protein scruin

Molecular Biology of the Cell
S SunPaul Matsudaira

Abstract

In the acrosomal process of Limulus sperm, the beta-propeller protein scruin cross-links actin into a crystalline bundle. To confirm that scruin has the topology of a beta-propeller protein and to understand how scruin binds actin, we compared the solvent accessibility of cysteine residues in scruin and the acrosomal process by chemical modification with (1,5-IAEDANS). In soluble scruin, the two most reactive cysteines of soluble scruin are C837 and C900, whereas C146, C333, and C683 are moderately reactive. This pattern of reactivity is consistent with the topology of a typical beta-propeller protein; all of the reactive cysteines map to putative loops and turns whereas the unreactive cysteines lie within the predicted interior of the protein. The chemical reactivities of cysteine in the acrosomal process implicate C837 at an actin-binding site. In contrast to soluble scruin, in the acrosomal process, C837 is completely unreactive while the other cysteines become less reactive. Binding studies of chemically modified scruin correlate the extent of modification at C837 with the extent of inhibition of actin binding. Furthermore, peptides corresponding to residues flanking C837 bind actin and narrow a possible actin-binding regio...Continue Reading

References

Dec 20, 1992·Journal of Molecular Biology·R ChattopadhyayaF A Quiocho
Sep 20, 1991·Journal of Molecular Biology·J N Varghese, P M Colman
May 5, 1984·Journal of Molecular Biology·D J DeRosier, L G Tilney
Apr 1, 1980·Experimental Cell Research·D J DeRosier, K T Edds
Mar 11, 1994·Journal of Molecular Biology·P Bork, R F Doolittle
Oct 1, 1993·The Journal of Cell Biology·C Owen, D DeRosier

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Citations

Aug 26, 1998·Folia Microbiologica·M Way
Jun 8, 2001·Progress in Biophysics and Molecular Biology·M Paoli
Dec 22, 1999·Trends in Cell Biology·J AdamsL Cooley
Aug 4, 2007·Science·Colin P JohnsonDennis E Discher
Jul 5, 2008·Proceedings of the National Academy of Sciences of the United States of America·Stanislav NagyRonald S Rock
Jun 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·J H ShinD A Weitz
Feb 21, 2002·The Journal of Cell Biology·Reed J KelsoLynn Cooley
Oct 20, 2006·The International Journal of Biochemistry & Cell Biology·Soren PragJosephine C Adams
Nov 14, 2002·Annual Review of Genetics·Andrew M Hudson, Lynn Cooley
Nov 11, 1999·Journal of Molecular Biology·M B ShermanM F Schmid
Nov 25, 2000·Biology of Reproduction·C LécuyerR Rousseaux-Prévost
Apr 18, 2002·FEBS Letters·Davy T'JampensJan Gettemans
Jan 4, 2020·Angewandte Chemie·Chang-Bo HuangPaolo Samorì
Nov 2, 2019·The Journal of Clinical Investigation·Josine M de WinterCoen Ac Ottenheijm
Apr 9, 2002·Structure·Zahra Jawad, Massimo Paoli
Feb 17, 1999·Biochimica Et Biophysica Acta·M Van TroysC Ampe
Feb 7, 2001·Science·L Mahadevan, P Matsudaira

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