PMID: 9536278Apr 16, 1998Paper

Modification of cystatin C activity by bacterial proteinases and neutrophil elastase in periodontitis

Molecular Pathology : MP
M AbrahamsonA Hall

Abstract

To study the interaction between the human cysteine proteinase inhibitor, cystatin C, and proteinases of periodontitis associated bacteria. Gingival crevicular fluid samples were collected from discrete periodontitis sites and their cystatin C content was estimated by enzyme linked immunosorbent assay (ELISA). The interaction between cystatin C and proteolytic enzymes from cultured strains of the gingival bacteria Porphyromonas gingivalis, Prevotella intermedia, and Actinobacillus actinomycetemcomitans was studied by measuring inhibition of enzyme activity against peptidyl substrates, by detection of break down patterns of solid phase coupled and soluble cystatin C, and by N-terminal sequence analysis of cystatin C products resulting from the interactions. Gingival crevicular fluid contained cystatin C at a concentration of approximately 15 nM. Cystatin C did not inhibit the principal thiol stimulated proteinase activity of P gingivalis. Instead, strains of P gingivalis and P intermedia, but not A actinomycetemcomitans, released cystatin C modifying proteinases. Extracts of five P gingivalis and five P intermedia strains all hydrolysed bonds in the N-terminal region of cystatin C at physiological pH values. The modified cystati...Continue Reading

References

Aug 30, 1991·Biochemical and Biophysical Research Communications·B R RifkinM Zimmerman
Sep 1, 1990·Scandinavian Journal of Clinical and Laboratory Investigation·D J ButtleM Abrahamson
Mar 1, 1990·Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Für Klinische Chemie Und Klinische Biochemie·D GabrijelcicV Turk
Jul 1, 1988·Journal of Periodontal Research·H Birkedal-HansenM E Neiders
Sep 1, 1986·Journal of Periodontal Research·T LahV Turk
Feb 1, 1986·Infection and Immunity·M Wikström, A Linde
Jan 1, 1974·Acta Odontologica Scandinavica·K OhlssonG Tynelius-Bratthall
Jul 1, 1970·European Journal of Biochemistry·S BlumbergA Berger
Apr 30, 1984·Biochemical and Biophysical Research Communications·A J BarrettA Grubb
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·A Grubb, H Löfberg
Jan 1, 1984·Experimental Cell Biology·P KöppelR Keller
Dec 14, 1984·Biochemical and Biophysical Research Communications·J M DelaisséG Vaes
Aug 1, 1984·Journal of Medical Microbiology·J CarlssonG K Sundqvist
Jan 1, 1994·Methods in Enzymology·M Abrahamson
Jan 1, 1997·Journal of Periodontal Research·J TravisJ Potempa
Aug 15, 1997·Journal of Molecular Biology·I EkielK Gehring

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Citations

Jul 20, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Bjarne VincentsMagnus Abrahamson
Feb 7, 2008·Journal of Periodontology·Taichi ItoTetsuo Kato
May 18, 2004·ANZ Journal of Surgery·Ming Khoon Yew, David Steinberg
Sep 24, 2008·Journal of Dental Research·R ElkaimH Tenenbaum
Dec 25, 2002·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·D P Dickinson
Dec 18, 2004·Critical Reviews in Clinical Laboratory Sciences·Michele Mussap, Mario Plebani
Jun 10, 2000·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·R J JohnE L Hewitt
Apr 25, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Xu YanXinwen Zhang
Sep 14, 1999·Oral Diseases·A BaronJ Featherstone
May 27, 2021·Genetic Testing and Molecular Biomarkers·Jeneffar Roselin ChristopherAnupama Tadepalli
Dec 5, 1998·Microbiology and Molecular Biology Reviews : MMBR·R J Lamont, H F Jenkinson

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