PMID: 9655530Jul 9, 1998Paper

Modification of enzymatic antioxidants in retinal microvascular cells by glucose or advanced glycation end products

Free Radical Biology & Medicine
C PagetM Lagarde

Abstract

Oxidative stress is one possible pathogenic mechanism to explain diabetic microangiopathy. In the present study, we determined the antioxidant enzyme activities in bovine retinal microvessels and cultured retinal microvascular cells: endothelial cells (BREC) and pericytes (BRP). We further investigated the effects of high glucose and advanced glycation end products (AGE) on these enzyme activities in BREC and BRP. Antioxidant enzyme activities in native retinal microvessels and BREC were quite similar but differed markedly from the BRP ones. High glucose decreased Se-GPx activity (about 20%) in BREC compared to mannitol. High concentrations of mannitol or NaCl increased Se-GPx activity (up to 40%) compared to control medium, suggesting that hyperosmolarity could regulate Se-GPx in BREC. No changes in antioxidant enzyme activities were observed when BRP were cultured with glucose or mannitol at high concentrations. AGE-BSA had no effect on enzyme activities in BREC, whereas 20 microM AGE-BSA increased catalase (40%) and superoxide dismutase (60%) activities in BRP. Differences in antioxidant enzyme activities observed between BREC and BRP, cultured with high concentrations of glucose or AGE, might help to explain their different...Continue Reading

References

Jun 1, 1979·Analytical Biochemistry·H OhkawaK Yagi
Jan 1, 1978·Methods in Enzymology·J D CrapoI Fridovich
Mar 14, 1978·Biochimica Et Biophysica Acta·S Pierce, A L Tappel
Nov 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·D GospodarowiczC Birdwell
Dec 1, 1975·Biochemical and Biophysical Research Communications·M O Hall, D O Hall
Mar 11, 1992·The American Journal of Physiology·F L SmardoA Garcia-Perez
Jan 1, 1991·Free Radical Biology & Medicine·D Armstrong, F al-Awadi
Jan 1, 1991·Annual Review of Medicine·M Brownlee
Feb 1, 1989·Experimental Eye Research·M I Naash, R E Anderson
Feb 1, 1988·Proceedings of the Society for Experimental Biology and Medicine·G M VercellottiC F Moldow
Dec 1, 1973·Analytical Biochemistry·W Schaffner, C Weissmann
Jul 1, 1995·Journal of Neurochemistry·T K MakarA J Cooper
Jan 1, 1994·Annual Review of Medicine·G L KingS E Bursell
Oct 18, 1995·Molecular and Cellular Biochemistry·R KakkarK Prasad
May 31, 1996·The Journal of Biological Chemistry·A ElgawishV M Monnier
Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·Y M LiH Vlassara
Jan 1, 1997·Diabetes Research and Clinical Practice·D Ruggiero-LopezN Wiernsperger
Jun 18, 1997·Biochemical and Biophysical Research Communications·N RellierN Wiernsperger

❮ Previous
Next ❯

Citations

Feb 6, 2004·BioFactors·N F Wiernsperger
Sep 18, 2012·Endocrine·Paulo MatafomeRaquel Seiça
Jul 2, 2003·Experimental and Molecular Pathology·Alan W Stitt
Mar 13, 2002·Atherosclerosis·Irit HochbergAndrew P Levy
Jan 12, 2010·Retina·Zvia Burgansky-EliashAdiel Barak
Jun 19, 2004·The British Journal of Ophthalmology·G GarhöferG T Dorner
Apr 2, 2009·Biological & Pharmaceutical Bulletin·Kwang Sik SuhYoung Seol Kim
Apr 5, 2008·Journal of Nutritional Science and Vitaminology·Hyun Young KimTakako Yokozawa
Feb 17, 2001·Biochemical and Biophysical Research Communications·A NatalizioN Wiernsperger
Sep 10, 2002·Proceedings of the National Academy of Sciences of the United States of America·Yoshito TanakaR Paul Robertson
Oct 23, 2002·Acta Ophthalmologica Scandinavica·Thomas A CiullaBruce Martin
Apr 16, 2010·Canadian Journal of Physiology and Pharmacology·Kaushik M DesaiLingyun Wu
Sep 28, 2010·Progress in Retinal and Eye Research·Chung-Jung Chiu, Allen Taylor
Jan 28, 2009·Pharmacotherapy·Tayyeba K Ali, Azza B El-Remessy
Jan 25, 2014·Scandinavian Journal of Clinical and Laboratory Investigation·Ina M KacsoCosmina I Bondor
Apr 20, 2004·Journal of Cellular and Molecular Medicine·A ManeaMonica Raicu
Jul 23, 2005·Annals of the New York Academy of Sciences·Alan W Stitt
Oct 6, 2005·Journal of Cellular and Molecular Medicine·Alina ConstantinDoina Popov
Nov 6, 2003·Redox Report : Communications in Free Radical Research·David M van ReykMichael J Davies
Aug 4, 2006·Endothelium : Journal of Endothelial Cell Research·A DuffyT O'Brien
Apr 5, 2011·Clinical Science·Drazenka Pongrac BarlovicKarin A M Jandeleit-Dahm
Sep 13, 2002·Expert Opinion on Investigational Drugs·Alan W StittMark E Cooper
Jun 7, 2005·Expert Opinion on Emerging Drugs·Grant M Comer, Thomas A Ciulla
Jul 4, 2012·Toxicology in Vitro : an International Journal Published in Association with BIBRA·B A GuerraR Otton
Jun 13, 2012·Journal of the Neurological Sciences·Kerstin BettermannMary E J Lott
Sep 17, 2016·Medicinal Research Reviews·Paulo MatafomeRaquel Seiça
May 1, 2004·Current Opinion in Ophthalmology·Thomas A CiullaMark H Criswell
Nov 18, 2016·The British Journal of Ophthalmology·Alexander D LinCecilia S Lee
Jul 12, 2003·Eye·M Donaldson, P M Dodson
Nov 4, 2004·Current Opinion in Ophthalmology·Grant M Comer, Thomas A Ciulla
Jul 9, 2005·Journal of Hypertension·Xiaoxia WangLingyun Wu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Brain Barrier

The blood brain barrier is a border that separates blood from cerebrospinal fluid. Discover the latest search on this highly selective semipermeable membrane here.