PMID: 9433914Jan 20, 1998Paper

Modification of glutamate-induced oxidative stress by lead: the role of extracellular calcium

Free Radical Biology & Medicine
J LoikkanenK M Savolainen

Abstract

The role of extracellular calcium in glutamate-induced oxidative stress, and the role of glutamatergic neuronal stimulation and oxidative stress in lead neurotoxicity were explored in mouse hypothalamic GT1-7 cells. Glutamate increased the production of reactive oxygen species (ROS) whether or not extracellular calcium was present. Glutamate-induced ROS production was amplified by lead acetate (PbAc), but only in the absence of extracellular calcium. However, PbAc on its own did not increase the production of ROS. A PKC inhibitor (Ro 31-8220) and superoxide dismutase (SOD) abolished the amplification of glutamate-induced production of ROS by PbAc, but did not inhibit ROS production induced by glutamate alone. Both glutamate and PbAc decreased the levels of intracellular glutathione (GSH), and amplified each other's effect on GSH depletion. Glutamate did not decrease cell viability, whereas the cytotoxicity of PbAc was amplified by glutamate. Extracellular calcium, a PKC inhibitor, or SOD did not modify the effects of glutamate, PbAc or their combination on the levels of GSH or cell viability. These data indicate that in GT1-7 cells extracellular calcium is not essential for glutamate-induced ROS production, which is amplified b...Continue Reading

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Citations

Nov 6, 2002·Journal of Biomedical Materials Research·J EngelmannW Geurtsen
Mar 31, 2004·Molecular Aspects of Medicine·Patricia I OteizaSandra V Verstraeten
Jul 10, 2003·Journal of Toxicology and Environmental Health. Part a·J NaaralaJ Liesivuori
Feb 20, 2014·Archives of Environmental Contamination and Toxicology·Fiorenza MinerviniAngelo Visconti
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Apr 26, 2011·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·P ErkekoğluF Hıncal
Dec 29, 2004·Free Radical Research·Helene Stockmann-JuvalaKai Savolainen
Mar 8, 2017·International Journal of Radiation Biology·Anne HöytöJonne Naarala
Feb 24, 2006·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·A FallareroP Vuorela
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Jun 28, 2005·Archives of Environmental Contamination and Toxicology·Nukhet Aykin-BurnsNuran Ercal
Feb 18, 2006·Toxicological Sciences : an Official Journal of the Society of Toxicology·Lucila Aimo, Patricia I Oteiza

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