Modification of macroporous titanium tracheal implants with biodegradable structures: tracking in vivo integration for determination of optimal in situ epithelialization conditions

Biotechnology and Bioengineering
Nihal Engin VranaPhilippe Lavalle

Abstract

Previously, we showed that macroporous titanium implants, colonized in vivo together with an epithelial graft, are viable options for tracheal replacement in sheep. To decrease the number of operating steps, biomaterial-based replacements for epithelial graft and intramuscular implantation were developed in the present study. Hybrid microporous PLLA/titanium tracheal implants were designed to decrease initial stenosis and provide a surface for epithelialization. They have been implanted in New Zealand white rabbits as tracheal substitutes and compared to intramuscular implantation samples. Moreover, a basement membrane like coating of the implant surface was also designed by Layer-by-Layer (LbL) method with collagen and alginate. The results showed that the commencement of stenosis can be prevented by the microporous PLLA. For determination of the optimum time point of epithelialization after implantation, HPLC analysis of blood samples, C-reactive protein (CRP), and Chromogranin A (CGA) analyses and histology were carried out. Following 3 weeks the implant would be ready for epithelialization with respect to the amount of tissue integration. Calcein-AM labeled epithelial cell seeding showed that after 3 weeks implant surfaces ...Continue Reading

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Citations

May 28, 2013·Tissue Engineering. Part B, Reviews·Nihal E VranaAli Khademhosseini
Sep 22, 2015·Regenerative Medicine·Hengyi ZhangZhiwei Xu
Apr 5, 2013·Journal of Biomedical Materials Research. Part a·Silvia LopaMatteo Moretti
Sep 9, 2017·Critical Reviews in Biotechnology·Julien BarthesNihal Engin Vrana
Jul 3, 2013·Advanced Healthcare Materials·Nihal Engin VranaPhilippe Lavalle
Feb 28, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·N E VranaP Lavalle

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