Modification of oil and glucosinolate content in canola seeds with altered expression of Brassica napus LEAFY COTYLEDON1

Plant Physiology and Biochemistry : PPB
Nosheen ElahiClaudio Stasolla

Abstract

Over the last few decades, research focusing on canola (Brassica napus L.) seed oil content and composition has expanded. Oil production and accumulation are influenced by genes participating in embryo and seed development. The Arabidopsis LEAFY COTYLEDON1 (LEC1) is a well characterized regulator of embryo development that also enhances the expression of genes involved in fatty acid (FA) synthesis. B. napus lines over-expressing or down-regulating BnLEC1 were successfully generated by Agrobacterium-mediated transformation. The constitutive expression of BnLEC1 in B. napus var. Polo, increased seed oil content by 7-16%, while the down-regulation of BnLEC1 in B. napus var. Topas reduced oil content by 9-12%. Experimental manipulation of BnLEC1 caused transcriptional changes in enzymes participating in sucrose metabolism, glycolysis, and FA biosynthesis, suggesting an enhanced carbon flux towards FA biosynthesis in tissues over-expressing BnLEC1. The increase in oil content induced by BnLEC1 was not accompanied by alterations in FA composition, oil nutritional value or glucosinolate (GLS) levels. Suppression of BnLEC1 reduced seed oil accumulation and elevated the level of GLS possibly through the transcriptional regulation of BnS...Continue Reading

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Citations

Jul 28, 2016·Plant Science : an International Journal of Experimental Plant Biology·Abdelhak FatihiLoïc Lepiniec
Sep 4, 2017·Plant Cell Reports·Youhong SongRay J Rose
Mar 28, 2019·Journal of Integrative Plant Biology·Leonardo JoJohn J Harada
Sep 17, 2016·Science·Patrick J Horn, Christoph Benning
Jun 1, 2016·Journal, Genetic Engineering & Biotechnology·Nosheen ElahiClaudio Stasolla

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