Modification of paper properties by the pretreatment of wastepaper pulp with Graphiumputredinis, Trichodermaharzianum and fusant xylanases

Bioresource Technology
S SavithaK Swaminathan

Abstract

Graphiumputredinis, Trichodermaharzianum and fusant were used in the present study to produce extracellular xylanases, an important industrial enzyme used in pulp and paper industry produced in a minimal medium supplemented with oat spelt xylan (1%, w/v) pH 7.0 at 27+/-2 degrees C. The enzyme was purified to homogeneity by DEAE-Cellulose and Superdex 75 FPLC column, respectively. The enzyme was found to be a monomer as determined by SDS gel electrophoresis. The optimum pH and temperature for purified G. putredinis, T. harzianum and fusant xylanases were 5.0-6.0 and 50-70 degrees C, respectively. Pretreatment of paper pulp with G. putredinis, T. harzianum and fusant xylanases decreased pulp kappa number. Xylanases particularly that of fusant at 5 IU/g pulp concentration and 1.5% pulp consistency at 60 degrees C for 18 h followed by EDED process yielded good quality paper from waste paper pulp. A significant increase in pulp brightness and improvement in various pulp properties, viz. burst capacity, thickness and bulkness of the treated pulp were observed in comparison to the conventional chemical bleaching. Easy purification and high stability of these enzymes makes it amicable for industrial applications.

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Citations

Aug 21, 2012·Applied Microbiology and Biotechnology·C E TorresA Blanco
Oct 28, 2009·Journal of Industrial Microbiology & Biotechnology·Sushil NagarRamesh Chander Kuhad
May 17, 2011·Applied Biochemistry and Biotechnology·Maíra Nicolau de AlmeidaSebastião Tavares de Rezende
Mar 29, 2016·Biotechnology for Biofuels·Vinay KhatriMarc Beauregard
Jan 17, 2012·Bioresource Technology·Sandra C T OliveiraJorge A Saraiva
Oct 11, 2014·Environmental Science and Pollution Research International·Cédric TarayrePhilippe Thonart

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