Modification of plasmid DNA topology by 'histone-mimetic' gold nanoparticles

Nanomedicine
João CondeJesus M de la Fuente

Abstract

Our aim is to explore whether gold nanoparticles (AuNPs) functionalized with a carboxylated polyethylene glycol (PEG) and protamine (AuNP@PEG@Prot) can modulate - enhance or restrain - DNA condensation, altering DNA conformation and inducing structural changes. Understanding how these nanoconjugates modulate DNA structure, size and shape of DNA condensates, and enable control over the resulting 3D structures is of major biological and therapeutic importance. Citrate-AuNPs were covered with a dense layer of a hetero-functional octa(ethylene glycol) (SH-EG(8)-COOH). Conjugation of protamine to the AuNP@PEG was achieved by taking advantage of the carboxylated surface previously generated on the surface of the NP and the remaining amino groups from the protamine, using carbodiimide and N-hydroxysulfosuccinimide coupling reactions. AuNP@PEG@Prot modulates the structure and topology of DNA, not only for condensation, but also for decondensation, via formation of higher quantities of dimers and multimers, when compared with AuNP@PEG and free protamine.

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Citations

Apr 8, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·João CondeFurong Tian
Oct 17, 2019·Frontiers in Bioengineering and Biotechnology·Mallikarjuna Rao GeddaPrasoon Madhukar

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Methods Mentioned

BETA
dynamic light scattering
electrophoresis
transfection
dynamic
infrared
Assay
atomic force microscopy
AFM

Software Mentioned

ImageJ
Nanotec WSxM

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