Modification of polystyrenic matrices for the purification of proteins. Effect of the adsorption of poly(vinyl alcohol) on the characteristics of poly(styrene-divinylbenzene) beads for use in affinity chromatography

Journal of Chromatography. a
D C NashH A Chase

Abstract

A poly(styrene-divinylbenzene) (PSDVB) chromatography matrix, CG1000-sd (TosoHaas), has been modified using poly(vinyl alcohol) (PVA) to create a matrix suitable for the attachment of functional groups for the selective purification of proteins. The characteristics of the modified matrix have been studied using a BET nitrogen adsorption/desorption technique and it has been found that the adsorption of PVA results in the bead micropores being filled whilst the bead macropores are left essentially unaltered. There was no protein adsorption onto the modified matrices. A dye ligand (Procion Blue MX-R) has been covalently attached to PVA-PSDVB matrix and the lysozyme capacities of the PVA-PSDVB matrix have been determined. The matrix compares well with commercial Blue Sepharose Fast Flow, an affinity matrix on cross-linked agarose. The dye-PVA-PSDVB matrix is stable when subjected to sanitisation with sodium hydroxide.

References

May 17, 1991·Journal of Chromatography·Y B Yang, F E Regnier
May 17, 1991·Journal of Chromatography·C P DesiletsF E Regnier
Feb 12, 1993·Journal of Chromatography·L VaradyF E Regnier

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Citations

Dec 23, 1999·Journal of Colloid and Interface Science·M LeonardE Dellacherie
Nov 6, 2001·Journal of Biochemical and Biophysical Methods·A Denizli, E Pişkin
Nov 1, 2012·Artificial Cells, Nanomedicine, and Biotechnology·Kazım Köse, Adil Denizli
Nov 5, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Jian-Bo QuGuang-Hui Ma
Nov 3, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Yali LiE T Kang

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