Modification of prolactin receptor (PRL-R) expression by PRL in the mouse liver: estimation of the ratio of two forms of PRL-R mRNAs by "one-sided competitive PCR"

Zoological Science
M Matsuda, T Mori

Abstract

We developed new means of measuring the ratio of the short to the long form (S/L ratio) of the mouse prolactin receptor (mPRL-R) cDNA by PCR using a primer common to the two forms and two specific primers. A means of estimating the amount of mPRL-R cDNA by competitive PCR was also established. We confirmed that these procedures were valid, since the S/L ratio of standard DNA was unaltered by one-sided cPCR amplification under the following conditions: the ratio was between 0.1 and 4, and the amount of cDNA was between 10(3) and 10(7) molecules/tube. The result of one-sided cPCR showed that the short form was dominant in the mouse liver, while the long form was dominant in other tissues. In addition, pituitary grafting increased the S/L ratio in the liver, implying that prolactin down-regulated the functional long form of PRL-R and lowered tissue sensitivity to prolactin itself by modifying the post-transcriptional regulation of PRL-R.

References

Oct 8, 1992·Nature·P D Siebert, J W Larrick
Oct 30, 1992·Biochemical and Biophysical Research Communications·M TanakaK Nakashima
Nov 27, 1991·Biochemical and Biophysical Research Communications·Z Z Hu, M L Dufau
Feb 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·L LesueurJ Djiane
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·G GillilandH F Bunn
Aug 1, 1990·Molecular Endocrinology·M ShirotaP A Kelly
May 28, 1990·Molecular and Cellular Endocrinology·L A Haldosén, J A Gustafsson
Apr 1, 1989·Molecular Endocrinology·J A Davis, D I Linzer
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M EderyJ Djiane
Mar 1, 1985·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·S SavoieB I Posner
Aug 1, 1994·General and Comparative Endocrinology·Y AkazomeS Kawashima
Jun 20, 1995·Proceedings of the National Academy of Sciences of the United States of America·O SandraP A Kelly
Aug 1, 1995·European Journal of Endocrinology·M MatsudaS Kawashima
Feb 1, 1994·European Journal of Endocrinology·M MatsudaS Kawashima

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BETA
PCR
electrophoresis

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