Modification of protein structures by altering the whey protein profile and heat treatment affects in vitro static digestion of model infant milk formulas

Food & Function
Amira HalabiAmélie Deglaire

Abstract

Heat treatments induce changes in the protein structure in infant milk formulas (IMFs). The present study aims to investigate whether these structural modifications affect protein digestion. Model IMFs (1.3% proteins), with a bovine or a human whey protein profile, were unheated or heated at 67.5 °C or 80 °C to reach 65% of denaturation, resulting in six protein structures. IMFs were submitted to in vitro static gastrointestinal digestion simulating infant conditions. During digestion, laser light scattering was performed to analyze IMF destabilization and SDS-PAGE, OPA assay and cation exchange chromatography were used to monitor proteolysis. Results showed that, during gastric digestion, α-lactalbumin and β-lactoglobulin were resistant to hydrolysis in a similar manner for all protein structures within IMFs (p > 0.05), while the heat-induced denaturation of lactoferrin significantly increased its susceptibility to hydrolysis. Casein hydrolysis was enhanced when the native casein micelle structure was modified, i.e. partially disintegrated in the presence of lactoferrin or covered by heat-denatured whey proteins. The IMF destabilization at the end of the gastric digestion varied with protein structures, with larger particle si...Continue Reading

References

Feb 26, 1991·European Journal of Biochemistry·A PierceG Spik
Mar 1, 1991·The Journal of Nutrition·W E HeineP J Reeds
May 1, 1989·Early Human Development·J R Britton, O Koldovský
Apr 1, 1985·Gastroenterology·N TobeyC Hoffner
Dec 1, 1973·Journal of the Science of Food and Agriculture·M G Davies, A J Thomas
Jun 1, 1967·Biochemistry·K Inouye, J S Fruton
Jan 1, 1982·Journal of Psychiatric Research·M W YogmanC Roberts
Dec 11, 2003·Journal of Agricultural and Food Chemistry·Fanny Guyomarc'hDouglas G Dalgleish
Feb 16, 2006·Journal of Agricultural and Food Chemistry·Magali LacroixClaire Gaudichon
Jun 15, 2006·Journal of Dairy Science·S M RutherfurdD Lowry
Jan 27, 2007·Journal of Pediatric Gastroenterology and Nutrition·James C KingRick de Waard
Apr 19, 2007·Journal of Agricultural and Food Chemistry·Laurence DonatoDouglas G Dalgleish
Sep 21, 2007·Journal of Dairy Science·G J O MartinD E Dunstan
Apr 11, 2008·The American Journal of Clinical Nutrition·Olof SandströmOlle Hernell
Jun 4, 2010·Molecular Nutrition & Food Research·Didier DupontAlan R Mackie
Oct 30, 2012·Journal of Dairy Science·Malleswara R PeramHarjinder Singh
Feb 16, 2013·Journal of Agricultural and Food Chemistry·Nicoleta StănciucMarc Hendrickx
May 11, 2013·The International Journal of Biochemistry & Cell Biology·Dereck E W ChattertonPer Torp Sangild
Jun 14, 2013·Biochemistry Research International·Sujata SharmaTej P Singh
Jul 3, 2013·Journal of Agricultural and Food Chemistry·Skelte G Anema, C G Kees de Kruif
Feb 18, 2015·Molecular Nutrition & Food Research·Gianluca PicarielloPasquale Ferranti
Jun 24, 2016·Nestlé Nutrition Institute Workshop Series·Bo Lönnerdal
Mar 20, 2019·Nature Protocols·André BrodkorbIsidra Recio
Mar 12, 2020·Food Research International·Linda Le RouxFrançoise Nau

❮ Previous
Next ❯

Methods Mentioned

BETA
light scattering
light
electrophoresis
confocal microscopy

Software Mentioned

Image Quant [UNK]
lawstat
nlme
lsmeans
MASS
R

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.