Modification of rice starch by selective degradation of amylose using alkalophilic Bacillus cyclomaltodextrinase

Journal of Agricultural and Food Chemistry
Joong-Hyuck AuhKwan-Hwa Park

Abstract

A cyclomaltodextrinase (CDase) isolated from alkalophilic Bacillus sp. I-5 (CDase I-5) exists in a dodecameric form, an assembly of six dimers, each catalytic site of which is located in a narrow groove at the interface of the dimeric unit. Because of the unique geometric shape of the catalytic site, the enzyme has the ability to discriminate the molecular size of substrates. An analysis of the hydrolysis reaction of the enzyme revealed that its kcat/Km value on amylose was 14.6 s(-1) (mg/mL)(-1), whereas that for amylopectin was 0.92 s(-1) (mg/mL)(-1), showing an exceptionally high preference toward amylose. CDase I-5 was applied to modify the starch structure to produce low-amylose starch products by incubating rice starch with this enzyme. We found that the amylose content of rice starch decreased from 28.5 to 9%, while the amylopectin content remained almost constant with no significant change in the side chain length distribution. When the CDase I-5-treated rice starch was stored at 4 degrees C for 7 days, the retrogradation rate was significantly retarded as compared to that in the control sample.

References

Feb 9, 2002·Biotechnology and Applied Biochemistry·Kyung-A CheongJung-Wan Kim
Mar 28, 2002·Applied and Environmental Microbiology·Hiroshi KamasakaTakashi Kuriki

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Citations

Jun 26, 2007·Bioscience, Biotechnology, and Biochemistry·Sung-Hoon ParkKwan-Hwa Park
Jan 24, 2007·Biotechnology Journal·Manuel FerrerPeter N Golyshin
Apr 25, 2020·Journal of Applied Microbiology·Pitchanan NimpiboonPiamsook Pongsawasdi
Oct 18, 2019·Journal of Nutritional Science and Vitaminology·Yusuke YamaguchiHitomi Kumagai
Sep 29, 2018·Food Science and Biotechnology·Sung Hoon ParkKwan-Hwa Park
Sep 1, 2018·Comprehensive Reviews in Food Science and Food Safety·Ming MiaoJames N BeMiller
Aug 28, 2018·Protein Expression and Purification·Fabiane Cristina Dos Santos, Ione Parra Barbosa-Tessmann

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