PMID: 9433469Jan 20, 1998Paper

Modification of short peptides using epsilon-aminocaproic acid for improved coating efficiency in indirect enzyme-linked immunosorbent assays (ELISA)

Journal of Immunological Methods
J C PyunJ S Park

Abstract

The hydrophobicity of short synthetic peptides of 5-10 residues was enhanced for high coating efficiency as antigens in indirect ELISA. To obtain enhanced hydrophobicity, coupling of epsilon-aminocaproic acids to the synthetic peptides was carried out during solid phase peptide synthesis. As a short peptide model, three analogues of a streptavidin binding peptide consisting of 5 amino acid residues were prepared with four epsilon-aminocaproic acid residues. HPLC analysis showed a dramatic increase in hydrophobicity after modification and the modified peptides showed a better adsorption ability than the unmodified peptides in indirect ELISA. The whole process from antigen coating to color development was carried out within 2.5 to 3 h by dissolving the peptide in methyl alcohol and evaporating the solvent in each well of the microplate. As an application of this method, a peptide assumed to function as one of the epitopes of the human 60 kDa Ro/SSA antigen was selected from hydrophilicity, acrophilicity and hydropathy plots. The peptide was synthesized having an epsilon-aminocaproic acid modification at both N and C terminal ends and was tested with 30 sera from patients with systemic lupus erythematosus (SLE), 20 normal sera and...Continue Reading

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Citations

Apr 12, 2000·Journal of Immunological Methods·M Steinitz, L Baraz
Feb 15, 2008·Journal of Clinical Microbiology·Sun-Hee ChoSun-Joong Kim
Mar 7, 2014·Applied Microbiology and Biotechnology·Xue Jun WangRong Yue Cao
Apr 11, 2000·Connective Tissue Research·R SrinivasanA George
Nov 20, 2015·The Analyst·Satish Balasaheb NimseTaisun Kim
Dec 2, 2009·Journal of Immunological Methods·Byung-Jin JeonJae-Chul Pyun
Jul 16, 2015·Journal of Pharmaceutical and Biomedical Analysis·Min ParkMin-Jung Kang
Jun 12, 2012·Journal of Immunological Methods·Maria Antonietta CuccuruPier Luigi Fiori

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