PMID: 6292915Nov 1, 1982Paper

Modification of single Na+ channels by batrachotoxin

Proceedings of the National Academy of Sciences of the United States of America
F N Quandt, T Narahashi

Abstract

The modifications in the properties of voltage-gated Na+ channels caused by batrachotoxin were studied by using the patch clamp method for measuring single channel currents from excised membranes of N1E-115 neuroblastoma cells. The toxin-modified open state of the Na+ channel has a decreased conductance in comparison to that of normal Na+ channels. The lifetime of the modified open state is drastically prolonged, and channels now continue to open during a maintained depolarization so that the probability of a channel being open becomes constant. Modified and normal open states of Na+ channels coexist in batrachotoxin-exposed membrane patches. Unlike the normal condition, Na+ channels exposed to batrachotoxin open spontaneously at large negative potentials. These spontaneous openings apparently cause the toxin-induced increase in Na+ permeability which, in turn, causes membrane depolarization.

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Citations

Mar 1, 1991·Pflügers Archiv : European journal of physiology·P NiemannW Ulbricht
Dec 1, 1995·Pflügers Archiv : European journal of physiology·H MotomuraN Ogata
Apr 1, 1989·Pflügers Archiv : European journal of physiology·K Chinn, T Narahashi
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