PMID: 2114251Jan 1, 1990Paper

Modification of skeletal S-1 with fluorescein isothiocyanate

Comparative Biochemistry and Physiology. B, Comparative Biochemistry
M J López-ZabalzaE Santiago

Abstract

1. Pyridoxal-5'-phosphate (PLP), a marker of primary amines, bound covalently to S-1 in an approximate ratio of 1:1. PLP was localized within the tryptic 25,000 mol. wt fragment. 2. Fluorescein isothiocyanate (FITC), which is known to bind covalently to primary amino groups located at nucleotide binding sites, readily bound to S-1 in a 2:1 ratio. FITC was localized within the 20,000 and 50,000 mol. wt tryptic peptides, in a ratio of 1:1 in each one. 3. These results are consistent with the existence of nucleotide binding sites on myosin different from those of the catalytic sites.

References

Oct 1, 1978·Archives of Biochemistry and Biophysics·M BálintF A Sréter
Apr 13, 1979·Biochemical and Biophysical Research Communications·L SzilagyiJ Gergely
Aug 13, 1979·Biochemical and Biophysical Research Communications·D MornetR Kassab
Apr 8, 1987·Biochimica Et Biophysica Acta·S SrivastavaS P Driska
Jan 1, 1987·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·M J López-ZabalzaE Santiago
Mar 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·Y Okamoto, R G Yount
Jan 30, 1986·Biochimica Et Biophysica Acta·A MuhlradT P Burghardt
Nov 15, 1973·Journal of Molecular Biology·U K Laemmli, M Favre
Sep 1, 1970·Biochimica Et Biophysica Acta·A Mühlrad, F Fábián
Sep 7, 1967·Biochemical and Biophysical Research Communications·A L ShapiroJ V Maizel
Feb 1, 1967·Biochemistry·E G RichardsH S Olcott
Dec 14, 1984·Biochemical and Biophysical Research Communications·T L KirleyL K Lane
Feb 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·A Muhlrad, M F Morales
Jun 15, 1983·Biochimica Et Biophysica Acta·R BernhardtK Ruckpaul
Jul 1, 1984·Journal of Biochemistry·T Hiratsuka
Dec 1, 1962·The Biochemical Journal·H MUELLER, S V PERRY

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.