PMID: 3758077Sep 15, 1986Paper

Modification of the actin interface of skeletal myosin subfragment-1 by treatment with dibromobimane

European Journal of Biochemistry
D MornetM F Morales

Abstract

Recently, by treating the head portion of skeletal myosin subfragment-1 (S1) with the bifunctional agent dibromobimane, we introduced an intramolecular covalent cross-link which resulted in the stabilisation of an internal loop in the heavy chain structure of the head [Mornet et al. (1984) Proc. Natl Acad. Sci. USA 82, 1658-1662]. In order to define the functional properties of this new S1 conformational state, we have first determined the experimental conditions for the optimum modification of S1 by dibromobimane. We finally settled on a 60% yield of cross-linked S1. Because the modification occurs between the 50-kDa and the 20-kDa tryptic heavy chain fragments which have been postulated to be involved in the interaction of native S1 with actin, we have investigated the association of dibromobimane-treated S1 with actin, using chemical cross-linking of their rigor complex with 1-ethyl-3-[3-(dimethylamino)propyl] carbodiimide. The cross-linked species obtained were analyzed by polyacrylamide gel electrophoresis and compared with those known for unmodified S1. The carbodiimide-catalyzed linkage between actin and dibromobimane-modified S1 led to a singlet protein band migrating with an apparent molecular mass of 155 kDa, in contr...Continue Reading

References

Aug 13, 1979·Biochemical and Biophysical Research Communications·D MornetR Kassab
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J A Wells, R G Yount
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·P ChaussepiedR Kassab
Mar 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D MornetM F Morales
Sep 15, 1973·Journal of Molecular Biology·F W Studier
Jul 23, 1981·Nature·D MornetR Kassab
Feb 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D MornetM F Morales
Apr 1, 1981·Biochimie·R KassabE Audemard

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Citations

Dec 4, 2009·Proceedings of the National Academy of Sciences of the United States of America·Kohji ItoKeiichi Yamamoto
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J BottsM F Morales
Apr 15, 1988·Biochemical and Biophysical Research Communications·A BonetD Mornet

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