PMID: 109110Jun 1, 1979

Modification of the immunogenicity and antigenicity of rat hepatoma cells. I. Cell-surface stabilization with glutaraldehyde

British Journal of Cancer
M R PriceR W Baldwin

Abstract

gamma-Irradiated rat hepatoma cells are immunogenic in syngeneic WAB/Not rats, so that immunized animals are protected against tumour-cell challenge and circulating tumour-specific antibody is produced. Treatment of the immunizing cells with glutaraldehyde at concentrations of 0.001% or greater for 30 min rendered these cells non-protective in tumour-rejection tests and no longer able to induce significant formation of specific antibody. However, tumour-specific antigens were shown to be expressed upon treated cells; they specifically bound tumour-specific antibody from syngeneic immune sera assessed in indirect membrane-immunofluorescence tests. Also, these cells specifically absorbed antibody from immune or tumour-bearer sera, as demonstrated in the indirect membrane-immunofluorescence test or a complement-dependent 51Cr-release test. Alloantigen expression was not influenced by glutaraldehyde treatment, although glutaraldehyde-treated hepatoma cells failed to induce alloantibody formation in KX/Not rats. Polyacrylamide-gel electrophoresis of treated cells, surface-labelled with 125I, indicated that extensive cross-linking of the surface protein occurred as a result of glutaraldehyde treatment. The present findings establish ...Continue Reading

Citations

Jan 1, 1982·Journal of Cancer Research and Clinical Oncology·K HöffkenC G Schmidt

Related Concepts

Antibody Formation
Tumor Antigens
Plasma Membrane
Chromium Radioisotopes
Anti-Human Globulin Consumption Test
SDS-PAGE
Immunofluorescence Assay
X-Rays, Nuclear
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Fab Immunoglobulins

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