Modification of the internal ribosome entry site element impairs the growth of foot-and-mouth disease virus in porcine-derived cells

The Journal of General Virology
Chao SunLi Yu

Abstract

The 5' untranslated region (5'UTR) of foot-and-mouth disease virus (FMDV) contains an internal ribosome entry site (IRES) that facilitates translation initiation of the viral ORF in a 5' (m7GpppN) cap-independent manner. IRES elements are responsible for the virulence phenotypes of several enteroviruses. Here, we constructed a chimeric virus in which the IRES of FMDV was completely replaced with that of bovine rhinitis B virus (BRBV) in an infectious clone of serotype O FMDV. The resulting IRES-replaced virus, FMDV(BRBV), replicated as efficiently as WT FMDV in hamster-derived BHK-21 cells, but was restricted for growth in porcine-derived IBRS-2, PK-15 and SK-6 cells, which are susceptible to WT FMDV. To identify the genetic determinants of FMDV underlying this altered cell tropism, a series of IRES-chimeric viruses were constructed in which each domain of the FMDV IRES was replaced with its counterpart from the BRBV IRES. The replication kinetics of these chimeric viruses in different cell lines revealed that the growth restriction phenotype in porcine-derived cells was produced after the replacement of domain 3 or 4 in the FMDV IRES. Furthermore, the change in FMDV cell tropism due to IRES replacement in porcine-derived cells...Continue Reading

References

Jan 1, 1990·Annual Review of Microbiology·A C Palmenberg
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R M del AngelV R Racaniello
Jul 1, 1968·Virology·L A Caliguiri, I Tamm
Apr 28, 1968·Journal of Molecular Biology·M F Jacobson, D Baltimore
May 1, 1994·Molecular Biology Reports·R J JacksonA Kaminski
Jan 1, 1993·Annual Review of Genetics·E WimmerX Cao
Mar 19, 1996·Proceedings of the National Academy of Sciences of the United States of America·M GromeierE Wimmer
May 17, 2000·The Journal of General Virology·J Schneider-Schaulies
Mar 20, 2001·Veterinary Research·F SobrinoV Ley
Apr 12, 2001·The Journal of General Virology·E Martínez-SalasS López de Quinto
Jul 11, 2001·Genes & Development·C U Hellen, P Sarnow
Oct 17, 2003·RNA·Olga Fernández-Miragall, Encarnación Martínez-Salas
Apr 16, 2004·Clinical Microbiology Reviews·Marvin J Grubman, Barry Baxt
Sep 29, 2004·Journal of Virology·Stephen GuestRaymond P Roos
Dec 8, 2004·Current Pharmaceutical Design·Encarnación Martínez-Salas, Olga Fernández-Miragall
May 3, 2007·Nature Reviews. Molecular Cell Biology·Bradley M LundeGabriele Varani
Aug 12, 2008·Virus Research·Olga Fernández-MiragallEncarnación Martínez-Salas
Feb 13, 2010·Journal of Biomedicine & Biotechnology·Almudena Pacheco, Encarnacion Martinez-Salas
Feb 11, 2011·Nucleic Acids Research·Yingpu YuChristopher U T Hellen
Nov 6, 2013·International Journal of Molecular Sciences·Encarnación Martínez-SalasRosa Diaz

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