Modification of the lipid moiety of the enterobacterial common antigen by the "Pseudomonas factor".

Infection and Immunity
H M KuhnH Mayer

Abstract

Pseudomonas aeruginosa produces a factor (PF) which affects the enterobacterial common antigen (ECA); resulting in failure of the antigen to modify erythrocytes for hemagglutination by ECA antibodies. In the present study the nature of PF was determined. Pronase treatment abolished its activity, indicating the protein nature of PF. PF-treated ECA no longer coated erythrocytes but still reacted with ECA antibodies in immunoelectrophoresis tests with monospecific antiserum to ECA, although differences were noted between the precipitation patterns of PF-treated and untreated ECA. Therefore, PF does not significantly affect the antigenic determinant of ECA but rather affects its lipid carrier, an L-glycerophosphatide. Accordingly, differences in the sugar chain could not be detected by high-voltage paper electrophoretic examinations of partial hydrolysates of PF-treated and untreated ECA. PF liberates all fatty acids from ECA, similarly to commercial lipases, as evidenced by the liberation of unsubstituted glycerol upon HF degradation at 0 degrees C of PF-treated ECA. The lipase activity of PF is indicated also by the observation that a strain of P. aeruginosa with reduced lipase production and an exolipase-negative strain affect E...Continue Reading

References

May 16, 1978·European Journal of Biochemistry·D Männel, H Mayer
May 16, 1978·European Journal of Biochemistry·D Männel, H Mayer
Nov 1, 1981·Infection and Immunity·H M KuhnH Mayer
May 1, 1965·Journal of Bacteriology·H Y WHANG, E NETER
Sep 9, 1950·Nature·W E TREVELYANJ S HARRISON

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Citations

Aug 1, 1991·Molecular Microbiology·M OhtaN Kato
Sep 1, 1988·FEMS Microbiology Reviews·H M KuhnH Mayer
Mar 6, 2003·Journal of Bacteriology·Paul J A ErbelKevin H Gardner
Oct 4, 2005·Journal of Bacteriology·Junko KajimuraPaul D Rick
Jan 2, 1987·European Journal of Biochemistry·H M KuhnH Mayer
Dec 11, 2013·The Journal of Biological Chemistry·Tomasz K GozdziewiczJolanta Lukasiewicz
Mar 13, 2014·FEMS Microbiology Reviews·Sergei KalynychMiroslaw Cygler
Aug 26, 2020·International Journal of Molecular Sciences·Anna MaciejewskaJolanta Lukasiewicz
Jan 16, 2021·International Journal of Molecular Sciences·Tomasz K GozdziewiczJolanta Lukasiewicz
Mar 24, 2021·Journal of Bacteriology·Nickolas G KesslerSean D Moore
May 1, 1985·Journal of Bacteriology·P D RickD Bitter-Suermann
Dec 1, 1986·Journal of Bacteriology·W StuerU K Winkler
Sep 1, 1993·Journal of Bacteriology·K Barr, P D Rick
Mar 1, 1985·Microbiological Reviews·H Nikaido, M Vaara
Aug 1, 1985·Journal of Bacteriology·U Meier, H Mayer

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