Modification of the method of thread manufacture improves stroke induction rate and reduces mortality after thread-occlusion of the middle cerebral artery in young or aged rats

Journal of Neuroscience Methods
Neil J SprattDavid W Howells

Abstract

Improving models of human stroke by the use of aged animals has been advocated; however the commonly used rat middle cerebral artery thread-occlusion model has produced suboptimal stroke induction and excess mortality in aged rats. We report the development of a modified method for silicone-coating the tip of occluding threads which produces a malleable silicone-coated tip which is firmly bonded and of highly consistent diameter, and overcomes problems of thread insertion through the narrowed carotid canal found in aged animals. Comparison of stroke outcomes and mortality were made between these threads and heat-treated poly-L-lysine coated threads. The rate of successful stroke induction in aged rats was significantly improved (from 14% to 86%). Similarly, mortality fell from 21-31% to 3-7% or less in both young and old rats with or without diabetes and hypertension. An occluding thread tip diameter of 0.35-0.38 mm was optimal for induction of mid-sized strokes in both young and old rats. This method of thread manufacture overcomes problems of inconsistency of diameter and bonding of the silicone-coated tip, and these threads produce significant improvements in stroke induction by MCA occlusion, particularly in aged animals an...Continue Reading

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Citations

May 23, 2012·Analytical and Bioanalytical Chemistry·Hay-Yan J WangCheng Bin Liu
Sep 25, 2012·Neurological Sciences : Official Journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·Ping YuanXiangyu Zhou
Nov 22, 2008·Stroke; a Journal of Cerebral Circulation·J Marc SimardVolodymyr Gerzanich
Jan 1, 2009·Journal of Experimental Stroke & Translational Medicine·Shimin LiuH Richard Winn
Jun 14, 2013·Cognitive, Affective & Behavioral Neuroscience·Matthew BoykoAlexander Zlotnik
May 20, 2011·Journal of Neurosurgical Anesthesiology·Matthew BoykoAlexander Zlotnik
Sep 19, 2012·Neuroscience·J LücklJ H Greenberg
Sep 10, 2011·Behavioural Brain Research·Anjali ChauhanYogendra Kumar Gupta
Mar 23, 2011·Behavioural Brain Research·Matthew BoykoAlexander Zlotnik
Feb 12, 2015·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Daniel J BeardNeil J Spratt
Aug 30, 2012·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Kathryn A SkeldingJohn A P Rostas
Aug 6, 2009·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Deepu R PillaiFelix Schlachetzki
Sep 24, 2009·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Asita S SarrafzadehMichail Plotkin
Jul 19, 2012·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Varun SharmaPeter J Wookey
Jan 14, 2010·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Sarah S J RewellDavid W Howells
Dec 18, 2014·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Lucy A MurthaNeil J Spratt
Apr 22, 2010·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Michelle J PorrittDavid W Howells
May 21, 2010·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·David W HowellsMalcolm R Macleod
Jun 21, 2012·International Journal of Stroke : Official Journal of the International Stroke Society·Sandeep AnkolekarPhilip M W Bath
Jul 5, 2012·International Journal of Stroke : Official Journal of the International Stroke Society·Emily S SenaDavid W Howells
May 26, 2011·International Journal of Stroke : Official Journal of the International Stroke Society·Damian D McLeodNeil J Spratt
Sep 13, 2013·International Journal of Stroke : Official Journal of the International Stroke Society·L A MurthaN J Spratt
Oct 22, 2013·International Journal of Stroke : Official Journal of the International Stroke Society·D D McLeodN J Spratt
Dec 14, 2011·International Journal of Stroke : Official Journal of the International Stroke Society·Fabien ChauveauMarlène Wiart
Jul 15, 2009·Brain Research·Neil J SprattDavid W Howells
Mar 22, 2014·Neurological Research·Timothy D KwiecienYuchuan Ding
Apr 30, 2014·Journal of Stroke and Cerebrovascular Diseases : the Official Journal of National Stroke Association·Xiaodong ChaoHua Han
Sep 15, 2012·Journal of Neuroscience Methods·Emily N J OrdLorraine M Work
Feb 7, 2015·Experimental & Translational Stroke Medicine·Amelia J TomkinsNeil J Spratt
Mar 23, 2011·Behavioural Brain Research·Baiba SvalbeMaija Dambrova

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