Modification of the microtubule-binding and ATPase activities of kinesin by N-ethylmaleimide (NEM) suggests a role for sulfhydryls in fast axonal transport.

Biochemistry
K K PfisterS T Brady

Abstract

N-Ethylmaleimide, an agent which alkylates free sulfhydryls in proteins, has been used to probe the role of sulfhydryls in kinesin, a motor protein for the movement of membrane-bounded organelles in fast axonal transport. When squid axoplasm is perfused with concentrations of NEM higher than 0.5 mM, organelle movements in both the anterograde and retrograde directions cease, and the vesicles remain attached to microtubules. Incubation of highly purified bovine brain kinesin with similar concentrations of NEM modifies the enzyme's microtubule-stimulated ATPase activity and promotes the binding of kinesin to microtubules in the presence of ATP. These results suggest that alkylation of sulfhydryls on kinesin alters the conformation of the protein in a manner that profoundly affects its interactions with ATP and microtubules. The NEM-sensitive sulfhydryls, therefore, may provide a valuable tool for the dissection of functional domains of the kinesin molecule and for understanding the mechanochemical cycle of this enzyme.

References

Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J A Wells, R G Yount
Jan 1, 1987·Cell Motility and the Cytoskeleton·K M JohnstonJ A Connolly
Mar 23, 1989·Nature·J M ScholeyL S Goldstein
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S A Kuznetsov, V I Gelfand
Sep 28, 1987·FEBS Letters·S M PenningrothD D Peterson
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·W M SaxtonJ R McIntosh
Sep 1, 1988·The Journal of Cell Biology·H S ShpetnerR B Vallee
Jan 1, 1987·Annual Review of Cell Biology·H M Warrick, J A Spudich
Nov 12, 1987·Nature·B M Paschal, R B Vallee
Aug 1, 1985·The Journal of Cell Biology·T A SchroerR B Kelly
Dec 13, 1972·Nature: New Biology·R SilvermanW W Kielley
Aug 1, 1980·The Journal of Cell Biology·M M Black, R J Lasek
Jan 1, 1983·Journal of Microscopy·R D Allen, N S Allen

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Citations

Jan 1, 1997·Cell Motility and the Cytoskeleton·R A WalkerM P Sheetz
Jun 1, 1995·Journal of Neurobiology·C WangK R Robinson
Jan 1, 1991·Pharmacology & Therapeutics·R F Luduena, M C Roach
Sep 13, 2002·Neurotoxicology·Dale W SicklesMarvin A Friedman
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S T BradyG S Bloom
Jul 1, 1991·The Journal of Cell Biology·N HirokawaS T Brady
Jun 10, 2000·Molecular Biology of the Cell·M Y TsaiS T Brady
Apr 1, 1997·Molecular Biology of the Cell·D L Stenoien, S T Brady
Oct 5, 1990·European Journal of Biochemistry·P K MaryaP A Eagles
Oct 1, 1992·Current Opinion in Neurobiology·T A Schroer
Jan 12, 2005·Nature Neuroscience·Dinara ShakiryanovaEdwin S Levitan
Jan 1, 1992·Cell Motility and the Cytoskeleton·P L LeopoldS T Brady
Oct 1, 1991·Neuron·S T Brady

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