PMID: 8939473Oct 25, 1996Paper

Modification of the N-methyl-D-aspartate (NMDA) receptor in the brain of newborn piglets following hyperventilation induced ischemia

Neuroscience Letters
E M GrahamM Delivoria-Papadopoulos

Abstract

The present study tests the hypothesis that cerebral ischemia induced by severe hypocapnia modifies the N-methyl-D-aspartate (NMDA) receptor/ion channel complex in the cerebral cortical cell membranes of newborn piglets. Studies were performed in six newborn piglets subjected to ischemic hypoxia induced by hyperventilation (PaCO2, 9-11 mmHg) for 1 h. Comparisons were made to a normoxic group on room air (n = 6). Following hyperventilation, phosphocreatine decreased 80%, but ATP remained unchanged. NMDA receptor activation was determined by measuring [3H]MK-801 binding at concentrations varying from 2.5 to 50 nM. Following hyperventilation, Bmax decreased 52% to 0.50 +/- 0.04 pmol/mg protein (P = 0.001); however, the Kd value was unchanged at 7.45 +/- 0.79 nM. Spermine and magnesium dependent activation of the NMDA receptor was determined in the hyperventilated and control groups. With spermine concentrations increasing from 2.5 to 50 microM the maximal spermine dependent activation in the normoxic group was 13.7 +/- 7.93% which occurred at a concentration of 3.75 +/- 1.37 microM. In the hyperventilated group maximal activation was 32.4 +/- 23.5% (P = 0.095) at 4.58 +/- 2.46 microM (P = ns). With magnesium concentrations increas...Continue Reading

References

Jan 1, 1992·Biology of the Neonate·J E DiGiacomoM Delivoria-Papadopoulos
Dec 1, 1992·Neurochemical Research·O P Mishra, M Delivoria-Papadopoulos
Dec 1, 1992·Neurochemical Research·O P Mishra, M Delivoria-Papadopoulos
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Citations

May 6, 1999·Brain Research Bulletin·O P Mishra, M Delivoria-Papadopoulos
Nov 1, 2008·Seminars in Fetal & Neonatal Medicine·Ulrich H Thome, Namasivayam Ambalavanan
Sep 5, 2006·Clinics in Perinatology·Karen I Fritz, Maria Delivoria-Papadopoulos
Apr 20, 2005·Journal of Human Hypertension·J E NaschitzI Rosner
Aug 5, 2017·Frontiers in Neurology·Daniel Agustín GodoyFrancisco Murillo-Cabezas
Feb 16, 2021·Frontiers in Neurology·Elisa Gouvea BogossianFabio Silvio Taccone

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