Modification of the response of a quiescent cell population within a murine solid tumour to boron neutron capture irradiation: studies with nicotinamide and hyperthermia

The British Journal of Radiology
S MasunagaM Abe

Abstract

C3H/He mice bearing SCC VII tumours received 5-bromo-2'-deoxyuridine (BrdU) continuously for 5 days via implanted mini-osmotic pumps, to label all proliferating (P) cells. 20 min after intraperitoneal injection of sodium borocaptate-10B (BSH), or 3 h after oral administration of dl-p-boronophenylalanine-10B (BPA), the tumours were irradiated with thermal neutrons. To modify the uptake dose of 10B, nicotinamide (NA) was intraperitoneally injected 60 min before the administration of 10B-compounds and/or the tumours were heated to 41.5 degrees C for 20 min immediately before irradiation. After irradiation, the tumours were excised, minced and trypsinized. The tumour cell suspensions were then incubated with cytochalasin-B (a cytokinesis-blocker). The micronucleus (MN) frequency in cells not BrdU-labelled (quiescent (Q) cells) was determined using immunofluorescence staining for BrdU. With or without the administration of 10B-compounds, the sensitivity of Q cells was lower than that of total (P + Q) tumour cells. With thermal neutron irradiation in the presence of either BPA or BSH, the MN frequency in each cell population was increased. A greater increase in the MN frequency of total tumour cells was observed after thermal neutron...Continue Reading

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Citations

Oct 31, 2007·International Journal of Radiation Oncology, Biology, Physics·Maria Alejandra DagrosaMario Alberto Pisarev
Oct 7, 2004·International Journal of Radiation Oncology, Biology, Physics·Shin-ichiro MasunagaKoji Ono
Jun 7, 2006·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Shin-Ichiro MasunagaKoji Ono
Aug 2, 2000·Japanese Journal of Cancer Research : Gann·S MasunagaH Hori
Sep 17, 1998·Japanese Journal of Cancer Research : Gann·S MasunagaM Akaboshi
Jun 12, 2002·Journal of Radiation Research·Shin-Ichiro Masunaga, Koji Ono

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