Modification of the susceptibility of gram-negative rods producing ESβLS to β-lactams by the efflux phenomenon

PloS One
Agnieszka E LaudyStefan Tyski

Abstract

The production of β-lactamases is the most important mechanism of Gram-negative rod resistance to β-lactams. Resistance to ceftazidime and cefepime in clinical isolates of Enterobacteriaceae (especially ESβL-positive E. coli and K. pneumoniae) and P. aeruginosa is life-threatening. However, all strains of the above mentioned species possess chromosomally encoded RND efflux pump systems in addition to β-lactamase production. The main goal of this study was to assess the role of efflux pump systems in cefepime and/or ceftazidime resistant phenotypes of ESβL-positive clinical strains of Enterobacteriaceae and P. aeruginosa. The influence of the efflux pump inhibitor PAβN on the minimum inhibitory concentration (MIC) values of tested cephalosporins was species-dependent. Generally, a significant reduction (at least four-fold) of β-lactam MICs was observed in the presence of PAβN only in the case of P. aeruginosa clinical isolates as well as the ESβL-producing transformant PAO1161 ΔampC. The usage of this agent resulted in the restoration of susceptibility to cefepime and/or ceftazidime in the majority of the P. aeruginosa ESβL-positive strains with low and moderate resistance to the above cephalosporins. Moreover, an outer membrane...Continue Reading

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Citations

Jan 14, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Agnieszka E LaudyStefan Tyski
Nov 9, 2017·Revista Do Instituto De Medicina Tropical De São Paulo·Marília Viana Albuquerque de AlmeidaRenata Albuquerque Costa
Apr 14, 2016·Antimicrobial Agents and Chemotherapy·Veronica N KosHumphrey A Gardner
Jul 18, 2018·Polish Journal of Microbiology·Agnieszka Ewa Laudy
Feb 1, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sabine SchusterWinfried V Kern
Jun 24, 2017·The Journal of Antimicrobial Chemotherapy·Anna BothHolger Rohde
Feb 27, 2021·Journal of Bioenergetics and Biomembranes·Fernando Gomes FigueredoMarta Maria de Franca Fonteles

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Methods Mentioned

BETA
PCR

Software Mentioned

Invitrogen
Vector NTI Advance TM 11

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