Modifications in cell cycle kinetics and in expression of G1 phase-regulating proteins in human amniotic cells after exposure to electromagnetic fields and ionizing radiation

Cell Proliferation
S LangeMyrtill Simkó

Abstract

Low-frequency electromagnetic fields are suspected of being involved in carcinogenesis, particularly in processes that could be related to cancer promotion. Because development of cancer is associated with deregulated cell growth and we previously observed a magnetic field-induced decrease in DNA synthesis [Lange et al. (2002) Alterations in the cell cycle and in the protein level of cyclin D1p, 21CIP1, and p16INK4a after exposure to 50 HZ. MF in human cells. Radiat. Environ. Biophys.41, 131], this study aims to document the influence of 50 Hz, 1 mT magnetic fields (MF), with or without initial gamma-ionizing radiation (IR), on the following cell proliferation-relevant parameters in human amniotic fluid cells (AFC): cell cycle distribution, expression of the G1 phase-regulating proteins Cdk4, cyclin D1, p21CIP1 and p16INK4a, and Cdk4 activity. While IR induced a G1 delay and a dose-dependent G2 arrest, no discernible changes in cell cycle kinetics were observed due to MF exposure. However, a significant decrease in the protein expression of cyclin D1 and an increase in p21CIP1- and p16INK4a-expression could be detected after exposure to MF alone. IR-exposure caused an augmentation of p21CIP1- and p16INK4a- levels as well, but d...Continue Reading

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Citations

May 28, 2011·Tissue Engineering. Part a·Cecilia OseraAlessia Pascale
Jun 29, 2010·Radiation and Environmental Biophysics·Ann-Christine MannerlingMats-Olof Mattsson
Sep 7, 2007·Osteoarthritis and Cartilage·C-H HsiehC-C Jiang
Sep 21, 2016·International Journal of Radiation Biology·Jukka LuukkonenJonne Naarala
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Apr 7, 2006·Journal of Cellular Biochemistry·Jana FrahmMyrtill Simkó
May 26, 2018·Proceedings. Biological Sciences·Jukka JuutilainenP J Hore

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