Modifications of intracellular Ca2+ signalling during nerve growth factor-induced neuronal differentiation of rat adrenal chromaffin cells

The European Journal of Neuroscience
N Jiménez, A Hernández-Cruz

Abstract

Postnatal sympathetic neurons (SNs) and chromaffin cells (CCs) derive from neural crest precursors. CCs can differentiate in vitro into SN-like cells after nerve growth factor (NGF) exposure. This study examines changes of intracellular Ca2+ homeostasis and dynamics of CCs under conditions that promote a neuronal phenotype. Spontaneous Ca2+ fluctuations, a frequent observation in early cultures of CCs, diminished after > 10 days in vitro in control cells and ceased in NGF-treated ones. At the same time, Ca2+ rises resulting from entry upon membrane depolarization, gradually increased both their size and peak d[Ca2+]i/dt, resembling those recorded in SNs. Concomitantly, caffeine-induced Ca2+ rises, resulting from Ca2+ release from intracellular stores, increased their size and their peak d[Ca2+]i/dt by > 1000%, and developed transient and sustained release components, similar to those of SNs. The transient component, linked to regenerative Ca2+ release, appeared after > 10 days of NGF treatment, suggesting a delayed steep enhancement of Ca2+-induced Ca2+ release (CICR). Immunostaining showed that proteins coded by the three known isoforms of ryanodine receptors (RyRs) are present in CCs, but that only RyR2 increased significantl...Continue Reading

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Citations

Dec 25, 2012·Molecules and Cells·Nammi ParkJae-Yong Park
Nov 15, 2002·The European Journal of Neuroscience·Ricardo RigualJavier Alvarez
Nov 26, 2009·Pharmacology & Therapeutics·Isaac N PessahPamela J Lein
Aug 8, 2006·Journal of Neurochemistry·Alvaro O ArdilesAna M Cárdenas
Oct 22, 2005·Journal of Neuroscience Research·Sang Min LeeJin Tae Hong
Jul 13, 2016·The Prostate·Andrew FarachGustavo E Ayala

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