Modifications of intracellular calcium release channels and calcium mobilization following 70% hepatectomy

Archives of Biochemistry and Biophysics
M Díaz-MuñozR Hernández-Muñoz

Abstract

The aim of this study was to investigate the properties of ryanodine and IP3 receptors in regenerating liver following 70% hepatectomy, and to evaluate the hepatic Ca2+ distribution and mobilization during this process. Specific [3H]ryanodine and [3H]IP3 binding to hepatic smooth endoplasmic reticulum membranes, as well as subcellular Ca2+ determination by atomic absorption flame photometry and Ca2+ mobilization by INDO-1 AM spectrofluorescence in hepatocytes, was performed in regenerating livers after surgical 70% hepatectomy. Incorporation of 14C amino acids into proteins and of 32P into phospholipids was done in subcellular fractions. Ryanodine receptor Kd presented a dramatic increase after 12 h of surgery and remained high up to 2 days of treatment. IP3 receptor Bmax showed a significant augmentation starting at 6 h after hepatectomy and returning to normal values after 1 week. Cytosolic total calcium content decreased from 12 h until 4 days after hepatectomy whereas the microsomal and mitochondrial total calcium increased at 1 and 2-4 days of liver regeneration, which coincided with the differential turnover of proteins and phospholipids in these fractions. ATP-induced Ca2+ transients in hepatocytes of 24-h-hepatectomized...Continue Reading

References

Feb 1, 1975·Analytical Biochemistry·U Fricke
Dec 1, 1975·Journal of Cellular Physiology·R H Rixon, J F Whitfield
Aug 4, 1992·Biochemical Pharmacology·B PereiraN Kraus-Friedmann
Jun 1, 1992·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·A KanekoT Kamada
Jun 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·A MiyawakiK Mikoshiba
Nov 1, 1990·Cell Calcium·N Kraus-Friedmann
Jun 15, 1990·The Biochemical Journal·V Shoshan-BarmatzN Kraus-Friedmann
Jul 1, 1986·Biochimie·J Sobczak, M Duguet
Dec 1, 1987·European Journal of Pharmacology·I Tsukamoto, S Kojo
Apr 1, 1967·The Biochemical Journal·P N CampbellG Serck-Hanssen
Jan 1, 1980·Annals of the New York Academy of Sciences·J F WhitfieldS H Swierenga
May 15, 1983·The Biochemical Journal·G Deliconstantinos, G Ramantanis
May 24, 1995·Molecular and Cellular Biochemistry·Y Kanayama, M Yamaguchi
Feb 1, 1995·Journal of Cellular Biochemistry·M Yamaguchi, Y Kanayama
Aug 1, 1994·Biological Trace Element Research·A MoritaY Itokawa
Nov 30, 1994·Biochemical and Biophysical Research Communications·L Feng, N Kraus-Friedmann
Apr 1, 1993·The Biochemical Journal·A Sanchez-Bueno, P H Cobbold
Sep 1, 1996·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·I Aguilar-DelfínR Hernández-Muñoz
Apr 4, 1997·Science·G K Michalopoulos, M C DeFrances

❮ Previous
Next ❯

Citations

Nov 3, 2012·International Journal of Hepatology·Isabelle Garcin, Thierry Tordjmann
Mar 9, 2011·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Julieta Díaz-Juárez, Rolando Hernández-Muñoz
Nov 28, 2006·Journal of Hepatology·Alexandra NicouThierry Tordjmann
Oct 18, 2000·Molecular Cell Biology Research Communications : MCBRC·F MagninoJ F Dufour
Apr 2, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Rolando Hernández-MuñozMyrna A R Dent
Mar 25, 2017·Oxidative Medicine and Cellular Longevity·Julieta A Díaz-Juárez, Rolando Hernández-Muñoz
Mar 11, 2003·Experimental Biology and Medicine·Ileana Hernández-RincónRolando Hernández-Muñoz
Aug 19, 2000·Laboratory Investigation; a Journal of Technical Methods and Pathology·R Hernández-MuñozO Vázquez-Martínez
Feb 20, 2007·Cancer Research·Béatrice Bailly-MaitreJohn C Reed
Mar 23, 2021·FEBS Letters·Chaonan JinJean-François Dufour
Sep 29, 2004·Cellular Signalling·Dong-Soon ImMichio Ui

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.