Modifications of neurofilament proteins by possible metabolites of allyl chloride in vitro

Drug and Chemical Toxicology
Y HeM Futatsuka

Abstract

Chronic exposure to allyl chloride (ALL) is known to produce a central-peripheral distal axonopathy. In relation to the mechanism(s), the present study was conducted to examine the abilities of ALL and its putative metabolites, i.e., epichlorohydrin, glycerol alpha-monochlorohydrin, allyl alcohol and acrolein to cross-link proteins in vitro. Neurofilament-riched cytoskeletal proteins (1mg/ml) and ovalbumin (10mg/ml) were incubated with 160 mM tested chemicals except for acrolein at 0.5 mM and 1 mM. Time-dependent studies by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) revealed that not only ALL, but also acrolein and epichlorohydrin exerted chemical modifications on axonal cytoskeletal proteins; while only acrolein-treated ovalbumin could manifest evidence of polymerization of the protein. Immunoblotting of PAGE-separated proteins confirmed that the high molecular weight proteins on the top of SDS-PAGEs were NF antigen-contained covalent cross-linked materials.

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Citations

Apr 20, 2007·Chemical Research in Toxicology·Keith P RomanoJulie T Millard
Apr 1, 2006·Biochemical Pharmacology·Nandita ShangariPeter J O'Brien
Aug 15, 2000·Journal of Structural Biology·S VaradarajanD A Butterfield
Oct 7, 2006·Toxicological Sciences : an Official Journal of the Society of Toxicology·Richard M LopachinSoma Das
Dec 13, 2005·Neurochemical Research·Qing-Shan WangSu-fang Yu
May 8, 2004·The Journal of Pharmacology and Experimental Therapeutics·Lisa M KaminskasPhilip C Burcham
Feb 4, 1999·Journal of Neurochemistry·N Y CalingasanG E Gibson

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