PMID: 7544629Aug 1, 1995Paper

Modified antisense oligodeoxynucleotides against the splice acceptor site of tat do not inhibit in vitro hematopoietic colony growth in HIV-positive patients

Annals of Hematology
R G GeisslerA Ganser

Abstract

The hematopoietic failure in the majority of patients with progressive HIV infection is further aggravated by virustatic agents like azidothymidine. As an alternative therapeutic attempt, three derivatives of an antisense oligodeoxynucleotide (ODN) against the splice acceptor site of the tat gene have been shown to inhibit HIV replication in vitro. This study was aimed at examining whether these agents are toxic to the hematopoietic progenitor cells. To this end, bone marrow cells from HIV-positive and healthy persons were depleted from adherent cells to eliminate fibroblasts. In further experiments, the cells were additionally enriched for CD34-positive hematopoietic progenitor cells or were depleted from delta TCS-1-positive T lymphocytes. At concentrations of 1.25-10 microM, the three antisense ODN did not inhibit any erythrocyte or granulocyte-monocyte colony growth from CD34-positive cells, either from the HIV-positive or from the HIV-negative cohort. In contrast to azidothymidine, which served as inhibitory control, a significant increase of colony growth was seen after depletion of fibroblasts, of delta TCS-1-positive cells, or without cell separation. In conclusion, the three oligodeoxynucleotides do not exert any hemat...Continue Reading

References

Aug 14, 1992·Biochemical and Biophysical Research Communications·G MauryR S Goody
Feb 4, 1987·Nature·H Mitsuya, S Broder
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D C MontefioriW M Mitchell
Jan 24, 1985·Nature·L RatnerK Baumeister
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S AgrawalP C Zamecnik
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R L LetsingerP S Sarin
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·B D WalkerJ Sodroski
Apr 25, 1988·Nucleic Acids Research·C A SteinJ S Cohen
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J GoodchildP C Zamecnik
Jan 22, 1993·Journal of Medicinal Chemistry·F MorvanJ L Imbach
May 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J LisziewiczS Agrawal

❮ Previous
Next ❯

Citations

Jun 2, 1998·The Journal of Hand Surgery : Journal of the British Society for Surgery of the Hand·M J Sandow

❮ Previous
Next ❯

Related Concepts

Related Feeds

Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.

Antisense Oligonucleotides: ND

This feed focuses on antisense oligonucleotide therapies such as Inotersen, Nusinursen, and Patisiran, in neurodegenerative diseases including amyotrophic lateral sclerosis.