Modified carbon nitride nanozyme as bifunctional glucose oxidase-peroxidase for metal-free bioinspired cascade photocatalysis

Nature Communications
Peng ZhangWonyong Choi

Abstract

Nanomaterials-based biomimetic catalysts with multiple functions are necessary to address challenges in artificial enzymes mimicking physiological processes. Here we report a metal-free nanozyme of modified graphitic carbon nitride and demonstrate its bifunctional enzyme-mimicking roles. With oxidase mimicking, hydrogen peroxide is generated from the coupled photocatalysis of glucose oxidation and dioxygen reduction under visible-light irradiation with a near 100% apparent quantum efficiency. Then, the in situ generated hydrogen peroxide serves for the subsequent peroxidase-mimicking reaction that oxidises a chromogenic substrate on the same catalysts in dark to complete the bifunctional oxidase-peroxidase for biomimetic detection of glucose. The bifunctional cascade catalysis is successfully demonstrated in microfluidics for the real-time colorimetric detection of glucose with a low detection limit of 0.8 μM within 30 s. The artificial nanozymes with physiological functions provide the feasible strategies for mimicking the natural enzymes and realizing the biomedical diagnostics with a smart and miniature device.

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Citations

Jan 30, 2020·Nanoscale·Jinyi Zhang, Juewen Liu
Feb 1, 2020·Preparative Biochemistry & Biotechnology·Ecem BaykalMelda Altikatoglu Yapaoz
May 19, 2020·The Analyst·Yufeng LiuHui Wei
May 28, 2020·Physical Chemistry Chemical Physics : PCCP·Xiang-Ming WangXu Han
May 22, 2020·The Analyst·Rabbee G MahmudunnabiMuhammad J A Shiddiky
Aug 12, 2020·Nanoscale Research Letters·Yonghua TangYouhui Lin
Jul 2, 2020·Nanoscale·Ievgen S DonskyiRainer Haag
Aug 28, 2020·Frontiers in Chemistry·Congcong Zhang, Xin Du
Jan 24, 2020·Frontiers in Chemistry·Yuanfeng LiHenk J Busscher
Sep 19, 2020·Physical Chemistry Chemical Physics : PCCP·Yawen TongWei Lin
Oct 21, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Bin LiuGang Wei
Jan 13, 2021·Sensors·Elicia L S WongEdith Chow
Apr 25, 2021·Advanced Materials·Weiqing XuChengzhou Zhu
Jun 8, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xiaoli LiuHui Wei
Jun 3, 2021·Biosensors·Ana R CardosoMaria Goreti Ferreira Sales
Jun 8, 2021·Chemical Science·Hongyu ZhangHua Kuang
Jun 23, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Lianlian FuYouhui Lin
Aug 23, 2019·Analytical Chemistry·Lei JiaoChengzhou Zhu
Jul 7, 2021·Small·Yu WuChengzhou Zhu

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Methods Mentioned

BETA
light scattering
X-ray
chip

Software Mentioned

Vienna Ab Initio Simulation Package ( VASP )

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