Modified citrus pectin inhibited bladder tumor growth through downregulation of galectin-3

Acta Pharmacologica Sinica
Tian FangRui-Min Huang

Abstract

Modified citrus pectin (MCP) is a carbohydrate enriched complex, which has been implicated in cancer treatment and prevention. However, the effects of MCP on urinary bladder cancer (UBC) are unknown. In this study, MCP was first tested in T24 and J82 human UBC cells and showed the inhibition of cell viability by the sulforhodamine B (SRB) assay. The MCP-treated UBC cells exhibited G2/M phase arrest with the decrease of Cyclin B1 and phosphorylated Cdc2. Caspase-3 was also activated, leading to the cleavage of Caspase-3 and PARP. We further explored the possible molecular mechanisms upon MCP treatment in UBC cells. Reduction of galectin-3 was observed and followed with the inactivation of Akt signaling pathway. Of note, galectin-3 knockdown by RNA interference recapitulated the MCP-mediated anti-proliferation, cell cycle arrest and apoptosis. Moreover, oral administration of MCP to the T24 xenograft-bearing nude mice inhibited the tumor growth significantly (P < 0.05). Quantification analysis of immunohistochemistry staining for Ki67 and cleaved Caspase-3 confirmed the decrease of proliferation index (P < 0.05) and the increase of apoptosis index (P < 0.01) in 700 mg/kg MCP-fed UBC xenografts. Using the information from TCGA dat...Continue Reading

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Citations

Jul 26, 2018·Integrative Cancer Therapies·Sefora ContiShahar Lev-Ari
Jan 16, 2020·Journal of Medicinal Food·Alejandra Bermúdez-OriaJuan Fernández-Bolaños
Apr 25, 2019·Nature Reviews. Urology·Neus Martínez-BoschPilar Navarro
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Feb 21, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Fanchao FengHailang He
Oct 17, 2020·European Journal of Pharmacology·Xin GuYangyang Xu

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Methods Mentioned

BETA
surgical resection
xenografts
protein assay
RNASeq
lavage
flow cytometry
FACS

Software Mentioned

GraphPad Prism
RNASeq V2 RSEM
FlowJo

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