Modified DOP-PCR for improved STR typing of degraded DNA from human skeletal remains and bloodstains

Legal Medicine
Angie AmbersBruce Budowle

Abstract

Forensic and ancient DNA samples often are damaged and in limited quantity as a result of exposure to harsh environments and the passage of time. Several strategies have been proposed to address the challenges posed by degraded and low copy templates, including a PCR based whole genome amplification method called degenerate oligonucleotide-primed PCR (DOP-PCR). This study assessed the efficacy of four modified versions of the original DOP-PCR primer that retain at least a portion of the 5' defined sequence and alter the number of bases on the 3' end. The use of each of the four modified primers resulted in improved STR profiles from environmentally-damaged bloodstains, contemporary human skeletal remains, American Civil War era bone samples, and skeletal remains of WWII soldiers over those obtained by previously described DOP-PCR methods and routine STR typing. Additionally, the modified DOP-PCR procedure allows for a larger volume of DNA extract to be used, reducing the need to concentrate the sample and thus mitigating the effects of concurrent concentration of inhibitors.

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Feb 18, 2014·Forensic Science International. Genetics·Angie AmbersBruce Budowle
May 6, 2014·International Journal of Legal Medicine·Angie AmbersBruce Budowle

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Citations

Mar 16, 2017·Forensic Science International. Genetics·Esiri TaskerSheree Hughes-Stamm
Aug 17, 2019·International Journal of Legal Medicine·Jeongyong KimSi-Keun Lim
May 31, 2018·International Journal of Legal Medicine·Michelle HarrelSheree Hughes-Stamm
Dec 8, 2019·Forensic Science International. Genetics·Man ChenJiangwei Yan
Mar 27, 2021·Clinica Chimica Acta; International Journal of Clinical Chemistry·Xue BaiZhiwei Zhang
Apr 22, 2021·International Journal of Legal Medicine·Katharina HelmWalther Gotsmy

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