Modified enzymatic procedure for the routine determination of glycerol and triglycerides in plasma.

A modification of Wieland's enzymatic procedure for glycerol analysis is presented. It is simple and precise, and readily applicable to the routine analysis of plasma and tissue glycerol and triglycerides. Optimal precision is obtained in samples containing 0.003-0.400 micromole of glycerol per ml, which in this method is equivalent to plasma levels of 0.011 to 1.40 micromoles/ml.