PMID: 2508308Jan 1, 1989Paper

Modified immunogold labelling applied to the study of protein droplets in glomerular disease

Virchows Archiv. A, Pathological Anatomy and Histopathology
M NakajimaP Kincaid-Smith

Abstract

Glomerular protein droplets have been observed in human glomerular epithelial cells, peripolar cells and mesangial cells, particularly in the preeclamptic lesion. Although morphologically similar, their functional role is uncertain. Whilst previous electron microscopic studies have shown these droplets to be generally uniform in electron density, our immunoelectron microscopy study of 10 biopsies, one showing lesions of preeclampsia, and the remaining 9 showing different forms of glomerular disease, generally displayed two distinct patterns of electron density and immunolabelling, when using a less severe fixation regime. An electron dense inner core was strongly positive to anti-fibrinogen, whilst a less dense peripheral zone was positive to anti-IgM. All other immunoglobulins, C3c, and albumin showed a less specific immunolabelling pattern. Protein droplets were also observed in proximal tubules in 7 out of 10 biopsies. In droplets found in most tubular cells immunolabelling was not partitioned, the most prominent findings being diffuse labelling for fibrinogen and albumin. In most instances where glomerular droplets were found adjacent to intraglomerular deposits, the marker identified within the deposit could also be seen i...Continue Reading

References

Jan 1, 1988·Virchows Archiv. A, Pathological Anatomy and Histopathology·I MorildA Bohle
Sep 1, 1987·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M BendayanF W Kan
Jan 1, 1988·Clinical and Experimental Hypertension. Part A, Theory and Practice·T HewitsonP Kincaid-Smith
Jan 1, 1986·Cell and Tissue Research·J A GallG B Ryan
Dec 1, 1974·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·I W McLean, P K Nakane
Jan 1, 1984·Clinical and Experimental Hypertension. Part A, Theory and Practice·P Kincaid-SmithR A Woods
Aug 1, 1980·Kidney International·H L Sheehan
Dec 15, 1963·American Journal of Obstetrics and Gynecology·C L PIRANIG FOLLI

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Citations

Jan 1, 1992·Virchows Archiv. A, Pathological Anatomy and Histopathology·D S GardinerG B Lindop
Jan 1, 1991·Virchows Archiv. A, Pathological Anatomy and Histopathology·M NakajimaP Kincaid-Smith
Feb 1, 1992·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·D S Gardiner, G B Lindop
Aug 1, 1992·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·D S GardinerG B Lindop
Feb 1, 1991·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·P Kincaid-Smith

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