Modified method for testing the quality of albumin-containing enrichments used in growth media for mycobacteria.

Journal of Clinical Microbiology
W R ButlerJ O Kilburn

Abstract

Many commercially available media for cultivation of mycobacteria have failed to support the growth of these organisms. This is especially true of media prepared with albumin-containing enrichments. Earlier, we developed a method for rapid identification of good albumin enrichments for agar-based media used to test the susceptibility of tubercle bacilli to pyrazinamide. The method was modified to make testing of the acceptability of albumin enrichments for primary isolation media for mycobacteria possible. We describe here a simple turbidimetric test using a specific Bacillus subtilis strain to assay quickly (24 h) different lots of albumin-containing enrichments that may be used in the preparation of growth media for mycobacteria.

References

Dec 1, 1986·Antimicrobial Agents and Chemotherapy·J J TarrandD H Gröschel
Feb 1, 1985·Journal of Clinical Microbiology·L B Heifets, M D Iseman
Dec 1, 1982·Journal of Clinical Microbiology·W R Butler, J O Kilburn

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Citations

Nov 8, 2016·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Marina de Azevedo IssaRômulo Cerqueira Leite
Oct 6, 2000·The Journal of Antimicrobial Chemotherapy·P Bemer-MelchiorH B Drugeon
Dec 1, 1992·Journal of Clinical Microbiology·M B CoyleE C Böttger

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