Modified nanoporous titanium dioxide as a novel carrier for enzyme immobilization

Biosensors & Bioelectronics
Lingtian WuHong Xu

Abstract

ε-Poly-L-lysine (EPL)-modified mesoporous titanium dioxide (M-TiO2) was assembled through the electrostatic attraction between EPL and M-TiO2. Through modification, the M-TiO2 surface tends to form multilayered and complex architectures, which can be used as artificial matrices to change the microenvironment of carriers for enzyme immobilization. The modified M-TiO2 was characterized through scanning electron microscopy, Fourier transform infrared spectroscopy, zeta potential analysis, and thermogravimetric analysis. All of the immobilized enzymes with negative charges display strong storage stability, thermal stability, and good reusability. Results indicate that EPL can self-assemble onto the surface of M-TiO2 and form a considerable number of active coatings. Our results also demonstrate that this simple and novel method can be potentially used to immobilize negatively charged enzymes for biosensor applications.

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Citations

Feb 6, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jing ChenAiping Xiao
Jan 11, 2018·International Journal of Environmental Research and Public Health·Violetta BorelliGiuliano Zabucchi
Aug 26, 2017·Environmental Toxicology and Chemistry·Chang LiuXiaojie Chang
Oct 25, 2017·Bioprocess and Biosystems Engineering·Paula NicolásMaría Luján Ferreira
Jan 7, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Fauzan AmriBrian Yuliarto
Dec 2, 2019·International Journal of Biological Macromolecules·Xingyu LuLingtian Wu
Jan 27, 2018·International Journal of Biological Macromolecules·Faranak JafarianAhmadreza Khosropour

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